DYNLT3 Is Required for Chromosome Alignment During Mouse Oocyte Meiotic Maturation

CORC > 厦门大学 > 医学院－已发表论文

	DYNLT3 Is Required for Chromosome Alignment During Mouse Oocyte Meiotic Maturation
	Huang, Xin ; Wang, Hai-Long ; Qi, Shu-Tao ; Wang, Zhen-Bo ; Tong, Jing-Shan ; Zhang, Qing-Hua ; Ouyang, Ying-Chun ; Hou, Yi ; Schatten, Heide ; Qi, Zhong-Quan ; Sun, Qing-Yuan ; Wang HL(王海龙) ; Qi ZQ(齐忠权)
刊名	http://dx.doi.org/10.1177/1933719111401664
	2011
关键词	DYNEIN LIGHT-CHAIN CAPSID PROTEIN VP26 CYTOPLASMIC DYNEIN SPINDLE CHECKPOINT INTERMEDIATE-CHAIN KINETOCHORE DYNEIN BINDING DOMAINS HEAVY-CHAIN ADULT BRAIN TCTEX-1
英文摘要	Major state basic research program China [2011CB944501]; Dynein light chain, Tctex-type 3 (DYNLT3), is a member of the cytoplasmic dynein DYNLT light chain family and has been reported to have a potential role in chromosome congression in human mitosis. However, its role in mammalian meiosis is unclear. In this study, we examined its localization, expression, and functions in mouse oocyte meiosis. Immunofluorescent staining showed that DYNLT3 was restricted to the germinal vesicle and associated with kinetochores at the germinal vesicle breakdown stage, metaphase I and metaphase II. The expression level of DYNLT3 was similar at all meiotic stages. Depletion of DYNLT3 by antibody injection resulted in chromosome misalignment and decrease of the polar body extrusion rate. We further found that DYNLT3-depleted oocytes displayed kinetochore-microtubule detachments. Chromosome-spread experiments showed that depletion of DYNLT3 inhibited the metaphase-anaphase transition by preventing homologous chromosome segregation in meiosis I. Our data suggest that DYNLT3 is required for chromosome alignment and homologous chromosome segregation during mouse oocyte meiosis.
语种	英语
出版者	REPROD SCI
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93274]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Huang, Xin,Wang, Hai-Long,Qi, Shu-Tao,et al. DYNLT3 Is Required for Chromosome Alignment During Mouse Oocyte Meiotic Maturation[J]. http://dx.doi.org/10.1177/1933719111401664,2011.
APA	Huang, Xin.,Wang, Hai-Long.,Qi, Shu-Tao.,Wang, Zhen-Bo.,Tong, Jing-Shan.,...&齐忠权.(2011).DYNLT3 Is Required for Chromosome Alignment During Mouse Oocyte Meiotic Maturation.http://dx.doi.org/10.1177/1933719111401664.
MLA	Huang, Xin,et al."DYNLT3 Is Required for Chromosome Alignment During Mouse Oocyte Meiotic Maturation".http://dx.doi.org/10.1177/1933719111401664 (2011).