Rapid detection of rpoB and katG genes from the sputum of multidrug-resistant Mycobacterium tuberculosis by polymerase chain reaction (PCR)-direct sequencing analysis

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	Rapid detection of rpoB and katG genes from the sputum of multidrug-resistant Mycobacterium tuberculosis by polymerase chain reaction (PCR)-direct sequencing analysis
	Yang, Song ; Zhong, Min ; Zhang, Yaoting ; Wang, Yiwei ; Zhang YT(张耀亭)
刊名	http://dx.doi.org/10.5897/AJMR11.358
	2011-11-16
关键词	MOLECULAR CHARACTERIZATION RIFAMPIN RESISTANCE DRUG-RESISTANCE MUTATIONS CHINA DIAGNOSTICS STRATEGIES PCR
英文摘要	Fujian Province Youthful Technical Innovational Fund [2005J081]; Rapid diagnosis of multidrug-resistant Mycobacterium tuberculosis (MDR-TB) plays a role in guiding standardized treatment regimen. Traditional drug susceptibility (DST) testing takes about six to eight weeks. Therefore, the aim of the present study is to characterize the mutations in rpoB and katG genes from the sputum specimens of MDR-TB by using polymerase chain reaction (PCR)-direct sequencing analysis and to diagnose the MDR-TB as soon as earlier. The sensitivity and specificity of this molecular DST would be assessed further. Rpob and katG genes were detected directly from the clinical sputum specimens of inpatients with MDR-TB by PCR-direct sequencing. The sensitivity, specificity of rpoB and katG gene for 48 specimens was as follows: 95.8, 100% and 93.75, 97.9%, respectively. Our study demonstrated that PCR-direct sequencing analysis was a rapid, sensitive and specific molecular approach for the mutation detection of rpoB and katG genes that are associated with multidrug-resistance clinical sputum specimens within 48 h of receipt, which is more rapid than drug susceptibility testing after the bacillus culture.
语种	英语
出版者	AFR J MICROBIOL RES
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93244]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Yang, Song,Zhong, Min,Zhang, Yaoting,et al. Rapid detection of rpoB and katG genes from the sputum of multidrug-resistant Mycobacterium tuberculosis by polymerase chain reaction (PCR)-direct sequencing analysis[J]. http://dx.doi.org/10.5897/AJMR11.358,2011.
APA	Yang, Song,Zhong, Min,Zhang, Yaoting,Wang, Yiwei,&张耀亭.(2011).Rapid detection of rpoB and katG genes from the sputum of multidrug-resistant Mycobacterium tuberculosis by polymerase chain reaction (PCR)-direct sequencing analysis.http://dx.doi.org/10.5897/AJMR11.358.
MLA	Yang, Song,et al."Rapid detection of rpoB and katG genes from the sputum of multidrug-resistant Mycobacterium tuberculosis by polymerase chain reaction (PCR)-direct sequencing analysis".http://dx.doi.org/10.5897/AJMR11.358 (2011).