Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses

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	Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses
	Xiong, H-R ; Li, Q. ; Chen, W. ; Liu, D-Y ; Ling, J-X ; Liu, J. ; Liu, Y-J ; Zhang, Y. ; Yang, Z-Q ; Li Q(李晴)
刊名	http://dx.doi.org/10.1007/s10096-010-1134-5
	2011-05
关键词	LINKED-IMMUNOSORBENT-ASSAY NEPHROPATHIA-EPIDEMICA GENETIC-CHARACTERIZATION IMMUNOGLOBULIN-G HUBEI PROVINCE INFECTIONS ANTIBODIES PREVENTION DIAGNOSIS PUUMALA
英文摘要	National High Technology Research and Development 863 Program of China [2007AA02Z465]; National Natural Science Foundation of China (NSFC) [30770096]; State Key Laboratory of Virology [2007-05]; Hemorrhagic fever with renal syndrome (HFRS) is endemic in East Asia and Europe. This study was initiated to investigate the reactivity of antibodies in sera of Chinese HFRS patients with the recombinant nucleocapsid proteins (rNPs) of Hantaan virus (HTNV), Dobrava-Belgrade virus (DOBV), and Puumala virus (PUUV), which are the prevalent hantavirus strains in Europe. Forty-eight pairs of acute and convalescent sera were collected from HFRS patients in Hubei, China (1985-2002) and tested by indirect IgG, IgA, and IgM enzyme-linked immunosorbent assays with six rNPs of European hantaviruses as coated antigens, respectively. The results showed that the sensitivity of rNPs against IgG was HTNV-rNP > DOBV-rNP > PUUV-rNP, while the sensitivity against IgA was DOBV-rNP > HTNV-rNP > PUUV-rNP. Quantitative analysis revealed both acute and convalescent sera from HFRS patients predominantly exhibit high levels of IgA. Although PUUV-rNPs showed very weak reactivity to the three kinds of immunoglobulins in all samples, three pairs of sera unexpectedly cross-reacted strongly to all three PUUV-rNP subtypes. We first observe that HFRS patients' sera from Hubei Province show new prevalent characteristics of cross-reacting with PUUV-rNPs and continued high level of IgA in convalescent phase, as well as in China.
语种	英语
出版者	EUR J CLIN MICROBIOL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93239]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Xiong, H-R,Li, Q.,Chen, W.,et al. Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses[J]. http://dx.doi.org/10.1007/s10096-010-1134-5,2011.
APA	Xiong, H-R.,Li, Q..,Chen, W..,Liu, D-Y.,Ling, J-X.,...&李晴.(2011).Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses.http://dx.doi.org/10.1007/s10096-010-1134-5.
MLA	Xiong, H-R,et al."Specific humoral reaction of hemorrhagic fever with renal syndrome (HFRS) patients in China to recombinant nucleocapsid proteins from European hantaviruses".http://dx.doi.org/10.1007/s10096-010-1134-5 (2011).