A Novel Method for Determination of Pepsin Based on Fluorescence Recovery of Aluminium Tetrasulphonated Phthalocyanine-Bovine Serum Albumin Complex Used as Substrate

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	A Novel Method for Determination of Pepsin Based on Fluorescence Recovery of Aluminium Tetrasulphonated Phthalocyanine-Bovine Serum Albumin Complex Used as Substrate
	Tao Yun ; Huang Ping ; Deng Ya-Bin ; Li Dong-Hui ; Huang P(黄萍) ; Li DH(李东辉)
刊名	http://dx.doi.org/10.3724/SP.J.1096.2010.01781
	2010-12
关键词	GASTRIC-JUICE QUANTUM DOTS
英文摘要	It was found that the fluorescence of aluminium tetrasulphonated phthalocyanine(AlS(4)Pc), a red-region fluorescence probe, was extremely quenched by bovine serum albumin (BSA) in acidic media, but recovered dramatically after the addition of pepsin. Based on this phenomenon, a new method was developed for homogeneous determination of pepsin. Under the optimal conditions(pH 2.5, reaction temperature 50 degrees C and reaction time of 1 h), the linear range of assay was 0.04 - 4 mg/L and the detection limit was 20 mu g/L. The constructed method has been used to the analysis of pepsin tablet samples with satisfied results.
语种	英语
出版者	CHINESE J ANAL CHEM
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93191]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Tao Yun,Huang Ping,Deng Ya-Bin,et al. A Novel Method for Determination of Pepsin Based on Fluorescence Recovery of Aluminium Tetrasulphonated Phthalocyanine-Bovine Serum Albumin Complex Used as Substrate[J]. http://dx.doi.org/10.3724/SP.J.1096.2010.01781,2010.
APA	Tao Yun,Huang Ping,Deng Ya-Bin,Li Dong-Hui,黄萍,&李东辉.(2010).A Novel Method for Determination of Pepsin Based on Fluorescence Recovery of Aluminium Tetrasulphonated Phthalocyanine-Bovine Serum Albumin Complex Used as Substrate.http://dx.doi.org/10.3724/SP.J.1096.2010.01781.
MLA	Tao Yun,et al."A Novel Method for Determination of Pepsin Based on Fluorescence Recovery of Aluminium Tetrasulphonated Phthalocyanine-Bovine Serum Albumin Complex Used as Substrate".http://dx.doi.org/10.3724/SP.J.1096.2010.01781 (2010).