A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method | |
Hong, Guolin ; Liu, Yinhuan ; Chen, Wei ; Weng, Shaohuang ; Liu, Qicai ; Liu, Ailin ; Zheng, Daoxin ; Lin, Xinhua ; Hong GL(洪国粦) ; Chen W(陈卫) | |
刊名 | http://dx.doi.org/10.2147/IJN.S35177 |
2012 | |
关键词 | SEQUENCE-SPECIFIC DETECTION SECONDARY STRUCTURE FEMTOMOLAR DNA HYBRIDIZATION SENSOR SURFACE AMPLIFICATION STABILITY DYNAMICS KINETICS |
英文摘要 | National High Technology and Development of China (863 Project) [2012AA022604]; National Natural Science Foundation of China [20975021, 21175023, 81171668, 21275028]; Fujian Provincial University-Industry Cooperation Science and Technology Major Program [2010Y4003]; Foundation of Fujian Key Laboratory of Hematology [2009J1004]; Scientific Research Major Program of Fujian Medical University [09ZD013]; Natural Science Foundation of Fujian Province of China [2010J06011, 2012J01428]; Medical innovation subject of Fujian Province of China [2012-CX-30]; Science and Technology Project of Fuzhou City of China [2011-S-67-3]; A highly sensitive and selective method for amplified electrochemical detection for hairpin-stem-loop structured target sequences was developed based on the temperature regulation of DNA hybrids on a sandwich-type electrochemical DNA sensor. Multistep hybridization was applied to promote the hybridization efficiency of each section of sandwich structure. The results showed that both multistep and temperature-controlling hybridization techniques were both especially made to fabricate the sensor for the tendency of internal hybridization of target gene sequences. This strategy provides significantly enhanced hybridization efficiency and sequence specificity of electrochemical detection. |
语种 | 英语 |
出版者 | INT J NANOMED |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/93126] |
专题 | 医学院-已发表论文 |
推荐引用方式 GB/T 7714 | Hong, Guolin,Liu, Yinhuan,Chen, Wei,et al. A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method[J]. http://dx.doi.org/10.2147/IJN.S35177,2012. |
APA | Hong, Guolin.,Liu, Yinhuan.,Chen, Wei.,Weng, Shaohuang.,Liu, Qicai.,...&陈卫.(2012).A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method.http://dx.doi.org/10.2147/IJN.S35177. |
MLA | Hong, Guolin,et al."A sandwich-type DNA electrochemical biosensor for hairpin-stem-loop structure based on multistep temperature-controlling method".http://dx.doi.org/10.2147/IJN.S35177 (2012). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论