Nuclear expression of Twist promotes lymphatic metastasis in esophageal squamous cell carcinoma

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	Nuclear expression of Twist promotes lymphatic metastasis in esophageal squamous cell carcinoma
	Gong, Taiqian ; Xue, Zengfu ; Tang, Shanhong ; Zheng, Xiushan ; Xu, Guanghui ; Gao, Liucun ; Zhao, Guohong ; Hong, Liu ; Tang, Guangbo ; Zhang, Hongwei ; Wang, Ruwen ; Jiang, Yaoguang ; Fan, Daiming ; Xue ZF(薛增福)
刊名	http://dx.doi.org/10.4161/cbt.19851
	2012-06
关键词	GROWTH-FACTOR-C EPITHELIAL-MESENCHYMAL TRANSITION VEGF-C BREAST-CANCER COLORECTAL-CANCER OVEREXPRESSION INDUCTION INVASION LYMPHANGIOGENESIS MIGRATION
英文摘要	China Postdoctoral Science Foundation [20090461447]; National Natural Science Foundation of China [81172288]; Twist-1 protein (also called Twist) has been suggested to be involved in tumor epithelial-mesenchymal transition (EMT) related progression, however, the mechanism by which Twist promotes lymph node metastasis is not fully understood. In the present study, we found that nuclear Twist expression is clearly correlated with lymph node (LN) metastasis as determined by immunohistochemistry (IHC). A highly invasive EC109 cell subline, EC109-P, was established by repeated in vitro transwell isolations for the cell model. Immunofluorescence (IF) assay demonstrated that nuclear Twist expression was markedly higher in the highly invasive EC109-P cell line when compared with EC109 and EC9706 cells. Based on our cell model, the function and mechanism by which Twist regulates LN metastasis in ESCC was investigated. The results showed that the overexpression of Twist could significantly increase the invasion and VEGF-C expression of EC9706 cells, whereas the knockdown of Twist expression results in the opposite effects. This finding was further strengthened by the results of the analysis of co-expression of Twist and VEGF-C by IHC in ESCC clinical samples. In summary, our study indicates that nuclear Twist plays an important role in ESCC lymphatic metastasis by increasing the expression of VEGF-C. The combination of Twist and VEGF-C detection could be a reliable prediction of LN metastasis in ESCC.
语种	英语
出版者	CANCER BIOL THER
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93108]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Gong, Taiqian,Xue, Zengfu,Tang, Shanhong,et al. Nuclear expression of Twist promotes lymphatic metastasis in esophageal squamous cell carcinoma[J]. http://dx.doi.org/10.4161/cbt.19851,2012.
APA	Gong, Taiqian.,Xue, Zengfu.,Tang, Shanhong.,Zheng, Xiushan.,Xu, Guanghui.,...&薛增福.(2012).Nuclear expression of Twist promotes lymphatic metastasis in esophageal squamous cell carcinoma.http://dx.doi.org/10.4161/cbt.19851.
MLA	Gong, Taiqian,et al."Nuclear expression of Twist promotes lymphatic metastasis in esophageal squamous cell carcinoma".http://dx.doi.org/10.4161/cbt.19851 (2012).