Alteration of endocannabinoid system in human gliomas

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	Alteration of endocannabinoid system in human gliomas
	Wu, Xiyue ; Han, Lijun ; Zhang, Xiaolin ; Li, Long ; Jiang, Changzhen ; Qiu, Yan ; Huang, Rui ; Xie, Baoying ; Lin, ZhiXiong ; Ren, Jie ; Fu, Jin ; Qiu Y(邱彦) ; Ren J(任杰) ; Fu J(傅瑾)
刊名	http://dx.doi.org/10.1111/j.1471-4159.2011.07625.x
	2012-03
关键词	CANCER CELL-PROLIFERATION CB2 RECEPTOR EXPRESSION CANNABINOID RECEPTOR ENDOGENOUS CANNABINOIDS N-ACYLETHANOLAMINES MALIGNANT GLIOMAS BRAIN-TISSUE HUMAN BREAST IN-VIVO ANANDAMIDE
英文摘要	Fujian Health-Education Research Grant, china [WKJ2008-2-45]; Xiamen Science and Technology Key Program Grant, China [3502Z20100006]; Endocannabinoids are neuromodulatory lipids that mediate the central and peripheral neural functions. Endocannabinoids have demonstrated their anti-proliferative, anti-angiogenic and pro-apoptotic properties in a series of studies. In the present study, we investigated the levels of two major endocannabinoids, anandamide and 2-arachidonylglycerol (2-AG), and their receptors, CB1 and CB2, in human low grade glioma (WHO grade I-II) tissues, high grade glioma (WHO grade III-IV) tissues, and non-tumor brain tissue controls. We also measured the expressions and activities of the enzymes responsible for anandamide and 2-AG biosynthesis and degradation, that is, N-acylphosphatidylethanolamine-hydrolysing phospholipase D (NAPE-PLD), fatty acid amide hydrolase (FAAH), monoacylglycerol lipase (MGL), and diacylglycerol lipase-alpha (DGL), in the same samples. Liquid chromatographymass spectometry analysis showed that the levels of anandamide decreased, whereas the levels of 2-AG increased in glioma tissues, comparing to the non-tumor controls. The expression levels and activities of NAPE-PLD, FAAH and MGL also decreased in glioma tissues. Furthermore, quantitative-PCR analysis and western-blot analysis revealed that the expression levels of cananbinoid receptors, CB1 and CB2, were elevated in human glioma tissues. The changes of anandamide and 2-AG contents in different stages of gliomas may qualify them as the potential endogenous biomarkers for glial tumor malignancy.
语种	英语
出版者	J NEUROCHEM
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/93095]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Wu, Xiyue,Han, Lijun,Zhang, Xiaolin,et al. Alteration of endocannabinoid system in human gliomas[J]. http://dx.doi.org/10.1111/j.1471-4159.2011.07625.x,2012.
APA	Wu, Xiyue.,Han, Lijun.,Zhang, Xiaolin.,Li, Long.,Jiang, Changzhen.,...&傅瑾.(2012).Alteration of endocannabinoid system in human gliomas.http://dx.doi.org/10.1111/j.1471-4159.2011.07625.x.
MLA	Wu, Xiyue,et al."Alteration of endocannabinoid system in human gliomas".http://dx.doi.org/10.1111/j.1471-4159.2011.07625.x (2012).