Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery

CORC > 厦门大学 > 医学院－已发表论文

	Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery
	Zhang, H. Z. ; Li, X. M. ; Gao, F. P. ; Liu, L. R. ; Zhou, Z. M. ; Zhang, Q. Q. ; Zhang QQ(张其清)
刊名	http://dx.doi.org/10.3109/10717540903508979
	2010-01
关键词	ASSEMBLED HYDROGEL NANOPARTICLES SOLVENT DIFFUSION METHOD HYDROPHOBIZED PULLULAN BINDING-PROTEIN CELL-LINES IN-VITRO RECEPTOR CONJUGATE DOXORUBICIN PH
英文摘要	Major State Basic Research Program of China [933300]; Doctoral Fund of Ministry of Education of China [2006023050]; The purpose of this work was to develop a novel nano-carrier with targeting property to tumor. In this study, pullulan acetate (PA) was synthesized by the acetylation of pullulan to simplify the preparation technique of nanoparticles. Folic acid (FA) was conjugated to PA in order to improve the cancer-targeting activity. The products were characterized by proton nuclear magnetic resonance ((1)H NMR) spectroscopy. Epirubicin-loaded nanoparticles were prepared by a solvent diffusion method. The loading efficiencies and EPI content increased with the amount of triethylamine (TEA) increasing in some degree. FPA nanoparticles could incorporate more epirubicin than PA nanoparticles. The folate-modifed PA nanoparticles (FPA/EPI NPs) exhibited faster drug release than PA nanoparticles (PA/EPI NPs) in vitro. Confocal image analysis and flow cytometry test revealed that FPA/EPI NPs exhibited a greater extent of cellular uptake than PA/EPI NPs against KB cells over-expressing folate receptors on the surface. FPA/EPI NPs also showed higher cytotoxicity than PA/EPI NPs. The cytotoxic effect of FPA/EPI NPs to KB cells was inhibited by an excess amount of folic acid, suggesting that the binding and/or uptake were mediated by the folate receptor.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/71452]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Zhang, H. Z.,Li, X. M.,Gao, F. P.,et al. Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery[J]. http://dx.doi.org/10.3109/10717540903508979,2010.
APA	Zhang, H. Z..,Li, X. M..,Gao, F. P..,Liu, L. R..,Zhou, Z. M..,...&张其清.(2010).Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery.http://dx.doi.org/10.3109/10717540903508979.
MLA	Zhang, H. Z.,et al."Preparation of folate-modified pullulan acetate nanoparticles for tumor-targeted drug delivery".http://dx.doi.org/10.3109/10717540903508979 (2010).