| A Monoclonal Antibody Against the Catalytic Domain of PTP1B | |
| Zeng, Fanwei ; Ni, Erru ; Wang, Shengyu ; Lv, Sha ; Cao, Chang ; Zhang, Haipeng ; Lv, Haiyan ; Chen, Xiaoli ; Yan, Jianghua ; Luo, Fanghong ; Luo FH(罗芳洪) | |
| 2012-06 | |
| 关键词 | TYROSINE-PHOSPHATASE 1B OVEREXPRESSION OBESITY |
| 英文摘要 | Protein tyrosine phosphatase 1B (PTP1B), a member of the protein tyrosine phosphatase (PTP) family, plays a crucial role in metabolic signaling, with insulin and leptin signaling being well studied. New evidence indicates that PTP1B is also involved in cancer. In the present study, we report on the establishment of a monoclonal antibody specific for catalytic domain of PTP1B (PTP1Bc) generated through the hybridoma method. The monoclonal antibody is measured to have a titer of 4.1 x 10(6) against PTP1Bc in indirect ELISA. Western blot and immunofluorescent analyses indicated that this antibody can specifically combine native PTP1B in MDA-MB-231 and MDA-MB-453 cells. This monoclonal antibody against PTP1Bc can help enhance the understanding of PTP1B-related physiological and pathological mechanisms and may act as a therapeutic agent for diabetes, obesity, and cancer in the future.; National Natural Science Foundation of China [30973485, 81172970]; Science and Technology Foundation of Fujian Province of China [2011R1039-1] |
| 语种 | 英语 |
| 出版者 | MARY ANN LIEBERT INC |
| 内容类型 | 期刊论文 |
| 源URL | [http://dx.doi.org/10.1089/hyb.2011.0115]  |
| 专题 | 医学院－已发表论文 |
| 推荐引用方式 GB/T 7714 | Zeng, Fanwei,Ni, Erru,Wang, Shengyu,et al. A Monoclonal Antibody Against the Catalytic Domain of PTP1B[J],2012. |
| APA | Zeng, Fanwei.,Ni, Erru.,Wang, Shengyu.,Lv, Sha.,Cao, Chang.,...&罗芳洪.(2012).A Monoclonal Antibody Against the Catalytic Domain of PTP1B.. |
| MLA | Zeng, Fanwei,et al."A Monoclonal Antibody Against the Catalytic Domain of PTP1B".(2012). |
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