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	Localization and Expression of Zonula Occludins-1 in the Rabbit Corneal Epithelium following Exposure to Benzalkonium Chloride
	Chen, Wensheng ; Chen WS(陈文生) ; Hu, Jiaoyue ; Zhang, Zhenhao ; Chen, Lelei ; Xie, Hui ; Dong, Nuo ; Chen, Yongxiong ; Liu, Zuguo
	2012-07-18
关键词	END-POINT ANALYSIS TIGHT JUNCTION BARRIER FUNCTION CELL-PROLIFERATION OCULAR SURFACE ROSE-BENGAL PROTEIN DISRUPTION RESISTANCE ZO-1
英文摘要	Preservatives are a major component of the ophthalmic preparations in multi-dose bottles. The purpose of this study was to investigate the acute effect of benzalkonium chloride (BAC), a common preservative used in ophthalmic preparations, on the localization and expression of zonula occludens (ZO)-1 in the rabbit corneal epithelium in vivo. BAC at 0.005%, 0.01%, or 0.02% was topically applied to one eye each of albino rabbits at 5 min intervals for a total of 3 times. The contralateral untreated eyes served as controls. The following clinical indications were evaluated: Schirmer test, tear break-up time (BUT), fluorescein and rose Bengal staining. The structure of central cornea was examined by in vivo confocal microscopy, and the corneal barrier function was evaluated by measurement of corneal transepithelial electrical resistance and permeability to carboxy fluorescein. Whole mount corneas were analyzed by using fluorescence confocal microscopy for the presence of ZO-1, 2, occludin, claudin-1, Ki67 and cell apoptosis in the epithelium. The expression of ZO-1 in the corneal epithelium was also examined by western blot and reverse transcription-polymerase chain reaction analyses. Exposure to BAC resulted in higher rose Bengal staining scores while no significant changes in BUT, Schirmer and corneal florescein scores. It also induced corneal epithelial cell damage, dispersion of ZO-1 and ZO-2 from their normal locus at the superficial layer and disruption of epithelial barrier function. However, the amounts of ZO-1 mRNA and protein in the corneal epithelium were not affected by BAC treatment. Exposure to BAC can quickly impair the corneal epithelium without tear deficiency. BAC disrupts the tight junctions of corneal epithelium between superficial cells in the rabbit corneal epithelium in vivo.; National Basic Research Program of China (Project 973) [2011CB504606]; National Natural Science Foundation of China, Beijing, China [30973249, 81100638]; Natural Science Foundation of Fujian Province, China [2009J01201]; Technological innovation platform program of Fujian Province, China [2009J1013]
语种	英语
出版者	PUBLIC LIBRARY SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1371/journal.pone.0040893]
专题	医学院－已发表论文
推荐引用方式 GB/T 7714	Chen, Wensheng,Chen WS,Hu, Jiaoyue,et al. Localization and Expression of Zonula Occludins-1 in the Rabbit Corneal Epithelium following Exposure to Benzalkonium Chloride[J],2012.
APA	Chen, Wensheng.,陈文生.,Hu, Jiaoyue.,Zhang, Zhenhao.,Chen, Lelei.,...&Liu, Zuguo.(2012).Localization and Expression of Zonula Occludins-1 in the Rabbit Corneal Epithelium following Exposure to Benzalkonium Chloride..
MLA	Chen, Wensheng,et al."Localization and Expression of Zonula Occludins-1 in the Rabbit Corneal Epithelium following Exposure to Benzalkonium Chloride".(2012).

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