Characterization of Outer Membrane Proteins of Escherichia Coli in Response to Phenol Stress

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	Characterization of Outer Membrane Proteins of Escherichia Coli in Response to Phenol Stress
	Zhang, Dan-Feng ; Li, Hui ; Lin, Xiang-Min ; Wang, San-Ying ; Peng, Xuan-Xian ; Zhang DF(张德富)
刊名	http://dx.doi.org/10.1007/s00284-010-9786-z
	2011-03
关键词	PSEUDOMONAS-PUTIDA KT2440 GRAM-NEGATIVE BACTERIA QUANTITATIVE PROTEOMICS SOLVENT TOLERANCE ORGANIC-SOLVENTS IDENTIFICATION PURIFICATION MECHANISMS RESISTANCE INSIGHTS
英文摘要	973A'' [2006CB 101807]; RFDP [200805580038]; NSFC [40876076]; Guangdong NSF [7117645] This study was sponsored by 973'' (2006CB 101807), RFDP (200805580038), NSFC (40876076), Guangdong NSF key project (7117645).; Gram-negative bacteria are generally more tolerant to disinfectants than Gram-positive bacteria due to outer membrane (OM) barrier, but the tolerant mechanism is not well characterized. We have utilized comparative proteomic methodologies to characterize the OM proteins of E. coli K-12 K99+ in response to phenol stress and found that nine proteins were altered significantly. They were OM proteins OmpA, FadL, LamB, and OmpT, cytoplasmic-associated proteins AceA and EF-Tu, inner membrane protein AtpB, putative capsid protein Q8FewO, and unknown location protein Dps. They were reported here for the first time to be phenol-tolerant proteins. The alteration and functional characterization of the four OM proteins were further investigated using western blotting, genetically modified strains with gene deletion and gene complementation approaches. Our results characterized the functional OM proteins of E. coli in resistance to phenol, and provide novel insights into the mechanisms of bacterial disinfectant-tolerance and new drug targets for control of phenol-resistant bacteria.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/70923]
专题	信息技术－已发表论文
推荐引用方式 GB/T 7714	Zhang, Dan-Feng,Li, Hui,Lin, Xiang-Min,et al. Characterization of Outer Membrane Proteins of Escherichia Coli in Response to Phenol Stress[J]. http://dx.doi.org/10.1007/s00284-010-9786-z,2011.
APA	Zhang, Dan-Feng,Li, Hui,Lin, Xiang-Min,Wang, San-Ying,Peng, Xuan-Xian,&张德富.(2011).Characterization of Outer Membrane Proteins of Escherichia Coli in Response to Phenol Stress.http://dx.doi.org/10.1007/s00284-010-9786-z.
MLA	Zhang, Dan-Feng,et al."Characterization of Outer Membrane Proteins of Escherichia Coli in Response to Phenol Stress".http://dx.doi.org/10.1007/s00284-010-9786-z (2011).