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	The regulation of silkworm fibroin L chain production by miRNA-965 and miRNA-1926 in insect cells
	Huang, Yong ; Zou, Quan ; Zou Q(邹权) ; Song, Fei ; Wang, Xin ; Shen, Xing Jia
	2012-07
关键词	silkworm miRNAs targets functional identification
英文摘要	MicroRNAs (miRNAs) are an abundant class of approximately 22-nucleotide (nt)-short noncoding RNA molecules present in the genomes of all multicellular organisms that act through base pairing to partially complementary sequences of the 3'untranslated region (UTR) of targeted mRNAs. Using bioinformatic approach, we found that the 3' UTR of the Fibroin L chain (Fib-L) mRNA matches perfectly the nucleotides 2-8 at the 5' end of the miRNA-965 and miRNA-1926. These two miRNAs might act as regulators of Fib-L gene expression at the post-transcriptional level. To examine whether Fib-L is directly targeted by miRNA-965 and miRNA-1926 in vitro, miRNA expression vectors and target reporter vector with 3'UTR of Fib-L were constructed respectively. Two vectors were co similar to transfected into Sf21 cells. The luciferase assay showed that miRNA-965 and miRNA-1926 may down regulate the expression of Fib-L via complementary interaction with the target sites in 3'UTR.; Natural Science Foundation of China [31172266]; Doctoral Science Foundation of He Nan University of Science [09001578]
语种	英语
出版者	MAIK NAUKA/INTERPERIODICA/SPRINGER
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1134/S1068162012030168]
专题	信息技术－已发表论文
推荐引用方式 GB/T 7714	Huang, Yong,Zou, Quan,Zou Q,et al. The regulation of silkworm fibroin L chain production by miRNA-965 and miRNA-1926 in insect cells[J],2012.
APA	Huang, Yong,Zou, Quan,邹权,Song, Fei,Wang, Xin,&Shen, Xing Jia.(2012).The regulation of silkworm fibroin L chain production by miRNA-965 and miRNA-1926 in insect cells..
MLA	Huang, Yong,et al."The regulation of silkworm fibroin L chain production by miRNA-965 and miRNA-1926 in insect cells".(2012).

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