The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods

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	The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods
	Yu, Xianyong ; Liao, Zhixi ; Yao, Qing ; Liu, Heting ; Li, Xiaofang ; Yi, Pinggui ; Yu XY(于贤勇)
刊名	http://dx.doi.org/10.1016/j.saa.2013.08.103
	2014-01-24
关键词	SPECTRAL METHODS FLUORESCENCE BINDING FLAVONOIDS PROBE
英文摘要	Scientific Research Fund of Hunan Provincial Education Department [12K101]; National Natural Science Foundation of China [21172066]; Hunan Provincial Natural Science Foundation of China [11JJ2007]; Opening Project of State Key Laboratory of Physical Chemistry of Solid Surfaces (Xiamen University) [201309]; Aid program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province; The fluorescence and ultraviolet-visible (UV-Vis) spectroscopy were explored to study the interaction between Tropicamide (TA) and bovine serum albumin (BSA) at three different temperatures (292, 301 and 310 K) under imitated physiological conditions. The experimental results showed that the fluorescence quenching mechanism between TA and BSA was static quenching procedure. The binding constant (K-a), binding sites (n) were obtained. The corresponding thermodynamic parameters (Delta H, Delta S and Delta G) of the interaction system were calculated at different temperatures. The results revealed that the binding process is spontaneous, hydrogen binds and vander Waals were the main force to stabilize the complex. According to Forster non-radiation energy transfer theory, the binding distance between TA and BSA was calculated to be 4.90 nm. Synchronous fluorescence spectroscopy indicated the conformation of BSA changed in the presence of TA. Furthermore, the effect of some common metal ions (Mg2+, Ca2+, Cu2+, and Ni2+) on the binding constants between TA and BSA were examined. (C) 2013 Elsevier B.V. All rights reserved.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/92077]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Yu, Xianyong,Liao, Zhixi,Yao, Qing,et al. The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods[J]. http://dx.doi.org/10.1016/j.saa.2013.08.103,2014.
APA	Yu, Xianyong.,Liao, Zhixi.,Yao, Qing.,Liu, Heting.,Li, Xiaofang.,...&于贤勇.(2014).The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods.http://dx.doi.org/10.1016/j.saa.2013.08.103.
MLA	Yu, Xianyong,et al."The investigation of the interaction between Tropicamide and bovine serum albumin by spectroscopic methods".http://dx.doi.org/10.1016/j.saa.2013.08.103 (2014).