Hippocampal metabolomics using ultrahigh-resolution mass spectrometry reveals neuroinflammation from Alzheimer's disease in CRND8 mice

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	Hippocampal metabolomics using ultrahigh-resolution mass spectrometry reveals neuroinflammation from Alzheimer's disease in CRND8 mice
	Lin, Shuhai ; Liu, Hongde ; Kanawati, Basem ; Liu, Liangfeng ; Dong, Jiyang ; Li, Min ; Huang, Jiandong ; Schmitt-Kopplin, Philippe ; Cai, Zongwei ; Dong JY(董继扬)
刊名	http://dx.doi.org/10.1007/s00216-013-6825-1
	2013
关键词	TRANSGENIC MOUSE MODEL SWEDISH APP MUTATION AMYLOID DEPOSITION METABOLITE IDENTIFICATION SECRETASE CLEAVAGE FT-ICR INFLAMMATION DEFICITS
英文摘要	Hong Kong SAR [HKBU200310]; Hong Kong Baptist University [IRMC/12-13/1A]; German Academic Exchange Service (DAAD) [A/12/00412]; In the wake of genomics, metabolomics characterizes the small molecular metabolites revealing the phenotypes induced by gene mutants. To address the metabolic signatures in the hippocampus of the amyloid-beta (A beta) peptides produced in transgenic (Tg) CRND8 mice, high-field ion cyclotron resonance-Fourier transform mass spectrometry supported by LC-LTQ-Orbitrap was introduced to profile the extracted metabolites. More than 10,000 ions were detected in the mass profile for each sample. Subsequently, peak alignment and the 80 % rule followed by feature selection based on T score computation were performed. The putative identification was also conducted using the highly accurate masses with isotopic distribution by interfacing the MassTRIX database as well as MS/MS fragmentation generated in the LTQ-Orbitrap after chromatographic separation. Consequently, 58 differentiating masses were tentatively identified while up to 44 differentiating elemental compositions could not be biologically annotated in the databases. Nonetheless, of the putatively annotated masses, eicosanoids in arachidonic acid metabolism, fatty acid beta-oxidation disorders as well as disturbed glucose metabolism were highlighted as metabolic traits of A beta toxicity in Tg CRND8 mice. Furthermore, a web-based bioinformatic tool was used for simulation of the metabolic pathways. As a result of the obtained metabolic signatures, the arachidonic acid metabolism dominates the metabolic perturbation in hippocampal tissues of Tg CRND8 mice compared to non-Tg littermates, indicating that A beta toxicity functions neuroinflammation in hippocampal tissue and new theranostic opportunities might be offered by characterization of altered arachidonic acid metabolism for Alzheimer's disease.
语种	英语
出版者	SPRINGER HEIDELBERG
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/91894]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Lin, Shuhai,Liu, Hongde,Kanawati, Basem,et al. Hippocampal metabolomics using ultrahigh-resolution mass spectrometry reveals neuroinflammation from Alzheimer's disease in CRND8 mice[J]. http://dx.doi.org/10.1007/s00216-013-6825-1,2013.
APA	Lin, Shuhai.,Liu, Hongde.,Kanawati, Basem.,Liu, Liangfeng.,Dong, Jiyang.,...&董继扬.(2013).Hippocampal metabolomics using ultrahigh-resolution mass spectrometry reveals neuroinflammation from Alzheimer's disease in CRND8 mice.http://dx.doi.org/10.1007/s00216-013-6825-1.
MLA	Lin, Shuhai,et al."Hippocampal metabolomics using ultrahigh-resolution mass spectrometry reveals neuroinflammation from Alzheimer's disease in CRND8 mice".http://dx.doi.org/10.1007/s00216-013-6825-1 (2013).