Group aggregating normalization method for the preprocessing of NMR-based metabolomic data

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	Group aggregating normalization method for the preprocessing of NMR-based metabolomic data
	Dong, Jiyang ; Cheng, Kian-Kai ; Xu, Jingjing ; Chen, Zhong ; Griffin, Julian L. ; Dong JY(董继扬)
刊名	http://dx.doi.org/10.1016/j.chemolab.2011.06.002
	2011-10-15
关键词	PRINCIPAL COMPONENT ANALYSIS TIME-DOMAIN ALGORITHM 1D H-1-NMR DATA SPECTRAL NORMALIZATION METABONOMICS SPECTROSCOPY NOISE
英文摘要	Science Research Foundation of the Ministry of Health; United Fujian Provincial Health and Education Project for Tackling the Key Research [WKJ2008-2-36]; NSF of Fujian Province of China [2009J05087, 2009J01299]; Data normalization plays a crucial role in metabolomics to take into account the inevitable variation in sample concentration and the efficiency of sample preparation procedure. The conventional methods such as constant sum normalization (CSN) and probabilistic quotient normalization (PQN) are widely used, but both methods have their own shortcomings. In the current study, a new data normalization method called group aggregating normalization (GAN) is proposed, by which the samples were normalized so that they aggregate close to their group centers in a principal component analysis (PCA) subspace. This is in contrast with CSN and PQN which rely on a constant reference for all samples. The evaluation of GAN method using both simulated and experimental metabolomic data demonstrated that GAN produces more robust model in the subsequent multivariate data analysis, more superior than both CSN and PQN methods. The current study also demonstrated that some of the differential metabolites identified using the CSN or PQN method could be false positives due to improper data normalization. (C) 2011 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/69773]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Dong, Jiyang,Cheng, Kian-Kai,Xu, Jingjing,et al. Group aggregating normalization method for the preprocessing of NMR-based metabolomic data[J]. http://dx.doi.org/10.1016/j.chemolab.2011.06.002,2011.
APA	Dong, Jiyang,Cheng, Kian-Kai,Xu, Jingjing,Chen, Zhong,Griffin, Julian L.,&董继扬.(2011).Group aggregating normalization method for the preprocessing of NMR-based metabolomic data.http://dx.doi.org/10.1016/j.chemolab.2011.06.002.
MLA	Dong, Jiyang,et al."Group aggregating normalization method for the preprocessing of NMR-based metabolomic data".http://dx.doi.org/10.1016/j.chemolab.2011.06.002 (2011).