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	Fetal rat metabonome alteration by prenatal caffeine ingestion probably due to the increased circulatory glucocorticoid level and altered peripheral glucose and lipid metabolic pathways
	Liu, Yansong ; Xu, Dan ; Feng, Jianghua ; Feng JH(冯江华) ; Kou, Hao ; Liang, Gai ; Yu, Hong ; He, Xiaohua ; Zhang, Baifang ; Chen, Liaobin ; Magdalou, Jacques ; Wang, Hui
	2012-07-15
关键词	Caffeine Intrauterine growth retardation Metabonome Glucocorticoid Intrauterine programming
英文摘要	The aims of this study were to clarify the metabonome alteration in fetal rats after prenatal caffeine ingestion and to explore the underlying mechanism pertaining to the increased fetal circulatory glucocorticoid (GC). Pregnant Wistar rats were daily intragastrically administered with different doses of caffeine (0, 20, 60 and 180 mg/kg) from gestational days (GD) 11 to 20. Metabonome of fetal plasma and amniotic fluid on GD20 were analyzed by H-1 nuclear magnetic resonance-based metabonomics. Gene and protein expressions involved in the GC metabolism, glucose and lipid metabolic pathways in fetal liver and gastrocnemius were measured by real-time RT-PCR and immunohistochemistry. Fetal plasma metabonome were significantly altered by caffeine, which presents as the elevated alpha- and beta-glucose, reduced multiple lipid contents, varied apolipoprotein contents and increased levels of a number of amino acids. The metabonome of amniotic fluids showed a similar change as that in fetal plasma. Furthermore, the expressions of 11 beta-hydroxysteroid dehydrogenase 2 (11 beta-HSD-2) were decreased, while the level of blood GC and the expressions of 11 beta-HSD-1 and glucocorticoid receptor (GR) were increased in fetal liver and gastrocnemius. Meanwhile, the expressions of insulin-like growth factor 1 (IGF-1), IGF-1 receptor and insulin receptor were decreased, while the expressions of adiponectin receptor 2, leptin receptors and AMP-activated protein kinase alpha 2 were increased after caffeine treatment. Prenatal caffeine ingestion characteristically change the fetal metabonome, which is probably attributed to the alterations of glucose and lipid metabolic pathways induced by increased circulatory GC, activated GC metabolism and enhanced GR expression in peripheral metabolic tissues. (C) 2012 Elsevier Inc. All rights reserved.; National Natural Science Foundation of China [30830112, 81072709, 30672566]; Chinese Ministry of Education [V200801]; Specialized Research Fund for the Doctoral Program of Higher Education of China [20110141120055]; Hubei Province Science Foundation for Innovation Group [2009CDA079, 2001CDA042]; Wuhan University [3081015]
语种	英语
出版者	ACADEMIC PRESS INC ELSEVIER SCIENCE
内容类型	期刊论文
源URL	[http://dx.doi.org/10.1016/j.taap.2012.05.002]
专题	物理技术－已发表论文
推荐引用方式 GB/T 7714	Liu, Yansong,Xu, Dan,Feng, Jianghua,et al. Fetal rat metabonome alteration by prenatal caffeine ingestion probably due to the increased circulatory glucocorticoid level and altered peripheral glucose and lipid metabolic pathways[J],2012.
APA	Liu, Yansong.,Xu, Dan.,Feng, Jianghua.,冯江华.,Kou, Hao.,...&Wang, Hui.(2012).Fetal rat metabonome alteration by prenatal caffeine ingestion probably due to the increased circulatory glucocorticoid level and altered peripheral glucose and lipid metabolic pathways..
MLA	Liu, Yansong,et al."Fetal rat metabonome alteration by prenatal caffeine ingestion probably due to the increased circulatory glucocorticoid level and altered peripheral glucose and lipid metabolic pathways".(2012).

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