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	CIN患者血清HPV16病毒颗粒IgG1、IgG2亚类抗体变化及临床意义; The association between IgG1,IgG2 subclasses toward human papillomavirus16-like particles in the serum and cervical intraepithelial neoplasia
	韩玲 ; 李海荣 ; 张秉宜 ; 佐满珍 ; 薛月珍 ; 张军
	2009
关键词	宫颈上皮内瘤变 IgG1、IgG2亚类 酶联免疫吸附试验 cervical intraepithelial neoplasia IgG1 and IgG2 subclasses ELISA
英文摘要	背景与目的:免疫球蛋白g(IMMunOglObulIn g,Igg)是血清和细胞外液中含量最高的免疫球蛋白,其某个亚类的升高,可能与宫颈上皮内瘤变(CErVICAl InTrAEPITHElIAl nEOPlASIA,CIn)的发生、发展及转归有密切相关。然而对于CIn患者血清人乳头瘤病毒样颗粒(HuMAn PAPIllOMAVIruS VIruS-lIkE PArTIClES,HPV VlPS)Igg1及Igg2抗体变化目前尚不明确。本研究旨在探讨CIn患者血清中HPV16VlPS-Igg1、Igg2亚类抗体的变化及其与不同级别宫颈病变的关系。方法:采用ElISA法检测HPV感染患者HPV感染患者CIn患者及子宫平滑肌瘤或宫颈炎患者血清中HPV16VlPS-Igg和HPV16VlPS-Igg1、Igg2亚类抗体的表达水平。结果:患者血清中HPV16VlPS-Igg和Igg1抗体含量随宫颈病变级别增高而增加,差异有统计学意义(P<0.05)。对照组、HPV感染组和CInⅠ组中以Igg2抗体为主(Igg2/Igg1>1),分别为100.00%、87.50%和75.00%,而CInⅡ~Ⅲ组中仅为9.52%,与前3组相比,差异有统计学意义(P<0.05)。HPV16-dnA阳性组的HPV16VlPS-Igg、Igg1、Igg2阳性率及吸光度A值明显高于非HPV16-dnA阳性组,差异有统计学意义(P<0.05)。HPV16-dnA检测与血清HPV16VlPS-Igg抗体检测之间呈中度相关(r=0.531,P<0.05)。结论:宫颈癌前病变尤其是CInⅡ~Ⅲ患者血清HPV16VlPS-Igg及其亚类表达增高,可能与病毒持续时间、病变严重程度有关;机体感染HPV后,低级别宫颈病变组、对照组血清HPV16VlPS-Igg2抗体表达增高(Igg2/Igg1>1),可能与HPV清除和宫颈病变逆转有关。; Background and purpose:The maximum content of immunoglobulin G was found in serum and extracellular fluid.The increase of some immunoglobulin G subclasses may closely correlate with the occurrence,development and the outcome of cervical lesions.However,the changes of IgG subclasses toward human papillomavirus 16-like particles(HPV16VLPs) in the serum of patients are still under investigation.This paper aimed to study this issue and also analyze the relationship between the expression of IgG subclasses and different grades of cervical lesions.Methods:The expression of IgG subclasses in 32 human papillomavirus,30 cervical intraepithelial neoplasia(CINⅠ) ,43 CINⅡ-Ⅲ,24 hysteromyoma and chronic cervicitis were examined by ELISA.Results:The absorbance value of HPV16-IgG,IgG1 increased with the grade of CIN(P<0.05) .The IgG2 dominance(IgG2/IgG1 ratio>1) from control group was 100%,87.50% for HPV infection group,75% for CINⅠ group,compared with that from CINⅡ-Ⅲ patients(9.52%) (P<0.05) .The positive rate and absorbance value of HPV16-IgG,IgG1,IgG2 from HPV16-DNA positive group were significantly higher than those from non-HPV16-DNA positive group(P<0.05) .There was a moderate correlation between the HPV16-DNA testing and detection of HPV16-IgG.Conclusion:An increase of the expression of HPV16-IgG and its subclasses in the serum of the patients with cervical precancerous lesions,especially those with CINⅡ-Ⅲ,might be associated with duration of HPV infection and severity of cervical lesions.An increase of the IgG2 dominance(IgG2/IgG1>1) in serum from low grade cervical lesions group and normal control group,might indicate the clearance of HPV infection and the regression of cervical lesions.
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119667]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	韩玲,李海荣,张秉宜,等. CIN患者血清HPV16病毒颗粒IgG1、IgG2亚类抗体变化及临床意义, The association between IgG1,IgG2 subclasses toward human papillomavirus16-like particles in the serum and cervical intraepithelial neoplasia[J],2009.
APA	韩玲,李海荣,张秉宜,佐满珍,薛月珍,&张军.(2009).CIN患者血清HPV16病毒颗粒IgG1、IgG2亚类抗体变化及临床意义..
MLA	韩玲,et al."CIN患者血清HPV16病毒颗粒IgG1、IgG2亚类抗体变化及临床意义".(2009).

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