| 我国南方地区急性散发型戊型肝炎病毒基因分析; Gene analysis of acute sporadic HEV in the southern areas of China | |
| 黄如统 ; 李德荣 ; 李庆虹 ; 魏绍静 ; 廖绵初 ; 李晓萸 ; 刘敏霞 ; 夏小兵 ; 苑锡同 | |
| 1999 | |
| 关键词 | 戊型肝炎病毒 基因 病毒 聚合酶链反应 序列分析 Hepatitis E virus Viral gene Polymerase chain reaction Sequence analysis |
| 英文摘要 | 目的进一步证实我国南方地区存在戊型肝炎病毒（HEV）不同的基因序列。方法用逆转录套式聚合酶链反应（rT-nESTEdPCr）检测8例厦门地区急性散发性成型肝炎患者血清中的HEVrnA，其中2例阳性；测定了广州分离株g93—2株病毒第5代培养液的HEVrnA。对阳性PCr产物进行克隆、核苷酸序列分析。结果厦门株X-S1与广州g93-2株病毒核苷酸和氨基酸序列的同源性为992%和975%；厦门株（X-S1）和广州株（g93-2）与缅甸株（bur—121）和我国新疆株（87A）的核苷酸和氨基酸序列的同源性为79.9%和86.3%，与墨西哥株的同源性为774%和%.3%。结论经序列分析结果进一步证实我国南方地区确实存在一种不同的HEV基因型。; ON.ctiv.To investigate the existing different bine wnuences of tiepatitis E virus (HEV)in the southern areas of China.Methods Eight acute--phase sera of sporadic patients with hepatitis E inXiamen were tested by reverse transcription-- polymerase chain reaction(RT -- P C R ).Two cases of them werepositive.The fad pessagu culture of Guamphou G93--2 Strain of HEV was also detected by RT--PCR.Thepositive RT--PCR products underwent cloning and nucleotide wnuencing.R.sults The homology of nucleotide and the amino acid between Xiamen X-S1 and Guangzhou G93--2 strain are 99.2% and 97.5%.Thehomology of nucleotide and the amino acid wnuences of Xiamen Strain and GuangZhou strain are 79.9%and 86.3% compared with the Burmese Strain (Bur--121) and our country Xitoiang Strain (87A), and are77.4% and 86.3% against Median Strain.Conclu.ion The results suggest that there is a different grnotypeof HEV in the southern areas of China.; 总后卫生部基金!9608037 |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/119622]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 黄如统,李德荣,李庆虹,等. 我国南方地区急性散发型戊型肝炎病毒基因分析, Gene analysis of acute sporadic HEV in the southern areas of China[J],1999. |
| APA | 黄如统.,李德荣.,李庆虹.,魏绍静.,廖绵初.,...&苑锡同.(1999).我国南方地区急性散发型戊型肝炎病毒基因分析.. |
| MLA | 黄如统,et al."我国南方地区急性散发型戊型肝炎病毒基因分析".(1999). |
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