QQ客服 官方微博 反馈留言

	在胍溶液中文昌鱼酸性磷酸酶活性部位的构象变化与活力变化的关系; Activity and ConFormational Changes at the Active Site ofACPase From Branchiostoma belcheri (Gray) in Guanidine Solutions
	陈素丽 ; 力政军 ; 符乃阳 ; 颜思旭
	1997
关键词	文昌鱼酸性磷酸酶 盐酸胍 活性部位构象 活力 Branchiostoma belcheri APCase Gu HCl Active site conFormation Activity
英文摘要	盐酸胍中酸性磷酸酶活性部位构象变化与活力变化的关系，酶于0.6MOl/lgu-HCl中，其相对活力提高20%，当gu-HCl浓度增加到1.2~2.4MOl/l时，酶的相对活力，其活性部位紫外-可见光谱500MM处吸收峰及荧光发射光谱515MM处的发射峰值下降，同时测定了酶的变性与失活常数，也研究了酶的重折叠与复性的关系; Activity and conFormational changes at the active site of ACPase in the presence of diFFerent concentration of Gu HCl have been studied by using Fluorescence emission spectroscopy With 0.6 mol/L Gu HCl,the enzyme relative activity was increased by 20%.As Gu HCl concentration increases From 1.2 to 2.4 mol/L,the enzyme relative activity and the intensity in the Fluorescence emission peak at 515 nm were reduced .The constants of denaturation and inactivation of ACPase in Gu HCl have also been determined .Result indicates that the rate constant of denaturetion is greater than those of inactivation .The relationship between the reactivation and the reFolding of the enzyme has been investigated.
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119416]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	陈素丽,力政军,符乃阳,等. 在胍溶液中文昌鱼酸性磷酸酶活性部位的构象变化与活力变化的关系, Activity and ConFormational Changes at the Active Site ofACPase From Branchiostoma belcheri (Gray) in Guanidine Solutions[J],1997.
APA	陈素丽,力政军,符乃阳,&颜思旭.(1997).在胍溶液中文昌鱼酸性磷酸酶活性部位的构象变化与活力变化的关系..
MLA	陈素丽,et al."在胍溶液中文昌鱼酸性磷酸酶活性部位的构象变化与活力变化的关系".(1997).

个性服务

查看访问统计

相关权益政策

暂无数据

收藏/分享

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。