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辐照蚕豆成熟花粉的细胞学效应; CYTOLOGICAL EFFECT of γ RADIATION ON vicia Faba POLLEN
王侯聪 ; 杨善民 ; 郑涛
1997
关键词蚕豆花粉 辐照 超微结构 细胞学效应 半致死剂量 Pollen grain of Vicia Faba γ irradiation cytological eFFect semilethal dose ultrastructure
英文摘要用60COγ射线0~837gy辐照蚕豆成熟花粉,观察在人工培养下萌发花粉的显微、亚显微辐射细胞学效应。花粉的萌发率和精核畸形率随辐照剂量的增大而提高,精细胞进管率则相应下降。在超微结构上,93gy辐照组的萌发花粉内,细胞核和细胞器未见异常,279gy辐照组的花粉管内,线粒体空泡化,并存在不成熟质体,465gy以上剂量辐照,核膜破裂,精核破碎,花粉管内存在多泡小体,偶尔有光化铁蛋白在不成熟质体内堆积。研究结果表明,蚕豆花粉的半致死剂量为465gy。; To study the radiation cytological eFFect on pollen grain Function, Fresh pollen samples shed From Vicia Faba were exposed to 60 Co γ ray in doses ranging From 0 to 837 Gy,and immediately permitted to germinate in vitro. It was shown that with increasing dose of γ irradiation, both the rate of pollen germination and the rate of sperm nucleus that migrating into pollen tube were reduced, concurrently, the rate of aberrant nuclei was elevated.Cytological examination showed that Formation of the nuclei and organella in the pollen tube appeared to be normal by irradiation of 93 Gy, while with γ radiation of 279 Gy and up subcellular structure of the germinating pollen cells exhibited various degrees of distortion: mitochondria and immature plastids vacuolated, nuclear membrance disrupted, sperm nucleus Fractured, occasionally, and photo chemoferritin accumulated in the immature plastids.With the doses of above 465Gy, multivesicular bodies presented in the pollen tube, suggesting that 465 Gy could be marked as the semilethal dose.; 福建省自然科学基金
语种zh_CN
内容类型期刊论文
源URL[http://dspace.xmu.edu.cn/handle/2288/119356]  
专题生命科学-已发表论文
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王侯聪,杨善民,郑涛. 辐照蚕豆成熟花粉的细胞学效应, CYTOLOGICAL EFFECT of γ RADIATION ON vicia Faba POLLEN[J],1997.
APA 王侯聪,杨善民,&郑涛.(1997).辐照蚕豆成熟花粉的细胞学效应..
MLA 王侯聪,et al."辐照蚕豆成熟花粉的细胞学效应".(1997).
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