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	转录延伸相关因子P-TEFb、HEXIM1与Brd4在小鼠胃腺里的荧光免疫检测; Immunofluorescence Detection of Transcription Elongation Factor P-TEFb,HEXIM1 and Brd4 in Mouse Gastric Glands
	林大河 ; 王琦 ; 冉海莹 ; 丁凤
	2014-05-28
关键词	胃泌酸腺 P-TEFb HEXIM1 Brd4 gastric gland P-TEFb HEXIM1 Brd4
英文摘要	探讨了正性转录延伸因子b(P-TEfb)及其相关调节因子在小鼠胃中的表达情况及意义.以正常小鼠为材料,通过多聚甲醛固定并石蜡包埋,利用免疫荧光组织化学的方法检测小鼠胃中P-TEfb的2个主要组成部分细胞周期蛋白依赖性激酶9(Cdk9)和细胞周期蛋白T1(CyClInT1)以及正向调控P-TEfb的募集因子brd4、P-TEfb的活性抑制蛋白HEXIM1的表达状况.检测结果表明:Cdk9和CyClInT1在鼠胃泌酸腺的所有细胞中均有较强的信号;HEXIM1在胃腺的中部和底部以及胃肌间神经丛内信号强烈;而brd4在胃腺的底部信号强烈.上述成果为在胃泌酸腺中构建P-TEfb调节转录延伸的正常生理模型奠定了基础.; In this study,the expression of positive transcription elongation factor b(P-TEFb)and its associated regulatory factors in mouse stomach was investigated.The mouse gastric tissue was fixed by paraformaldehyde,embedded with paraffin,and analyzed by immunofluorescence assay.The results showed that CDK9and CyclinT1,two subunits of P-TEFb,were highly expressed in all cells of gastric glands.HEXIM1,a negative regulator for P-TEFb,was strongly expressed in cells located at the bottom of gastric gland and myenteric plexus.Brd4,apositive regulator for P-TEFb′s recruitment to the promoter region to activate gene transcription,was strongly expressed in cells at the bottom of gastric gland.These observations laid a foundation for the study of P-TEFb modulated gene expression in a normal tissue.; 国家自然科学基金(81070307); 福建省自然科学基金(2010J01231)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119292]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	林大河,王琦,冉海莹,等. 转录延伸相关因子P-TEFb、HEXIM1与Brd4在小鼠胃腺里的荧光免疫检测, Immunofluorescence Detection of Transcription Elongation Factor P-TEFb,HEXIM1 and Brd4 in Mouse Gastric Glands[J],2014.
APA	林大河,王琦,冉海莹,&丁凤.(2014).转录延伸相关因子P-TEFb、HEXIM1与Brd4在小鼠胃腺里的荧光免疫检测..
MLA	林大河,et al."转录延伸相关因子P-TEFb、HEXIM1与Brd4在小鼠胃腺里的荧光免疫检测".(2014).

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