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	离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性; Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography
	范艳群 ; 许建中 ; 徐询 ; 刘亿婕 ; 许晨
	2014-04-10
关键词	离子排斥色谱 葡萄糖醛酸 葡萄糖醛酸内酯 稳定性 ion-exclusion chromatography glucuronic acid glucuronolactone stability
英文摘要	建立了同时检测葡萄糖醛酸和葡萄糖醛酸内酯的离子排斥色谱法。采用bOSTOn HC-b75 H+色谱柱进行分离,以5 MMOl/l H2SO4水溶液为流动相,流速0.6 Ml/MIn,柱温25℃,紫外检测波长220 nM;根据外标法定量,葡萄糖醛酸和内酯分别在10~1200 Mg/l和5~600 Mg/l范围内线性良好(r>0.9999);葡萄糖醛酸、葡萄糖醛酸内酯的最低检测限及定量限均分别为0.1和0.3 Mg/l,精密度实验rSd分别为1.00%和0.35%,平均回收率分别为99.69%和98.21%。该检测方法简便、快速、灵敏,可用于葡萄糖醛酸和葡萄糖醛酸内酯的同时测定。温度、PH值和溶剂对葡萄糖醛酸稳定性有影响,可为葡萄糖醛酸的分析与制备研究提供参考依据。; An analytical method was established for the simultaneous determination of glucuronic acid and glucuronolactone using ion-exclusion chromatography.The separation was performed on a Boston HC-B75 H+ column at 25 ℃ by HPLC equipped with an ultraviolet detector at the wavelength of 220 nm.The mobile phase is 5 mmol / L sulfuric acid with a flow rate of 0.6 mL / min.The quantification was performed by an external standard.The linear ranges for glucuronic acid and glucuronolactone are 10 ~ 1200 mg / L and 5 ~ 600 mg / L( r > 0.9999),respectively.The detection and quantification limits for the two compounds are 0.1 mg / L and 0.3 mg / L,respectively.The standard deviations for glucuronic acid and glucuronolactone are 1.00% and 0.35%,respectively.The recovery ratios for glucuronic acid and glucuronolactone are 99.69% and 98.21%,respectively.This method is convenient,rapid,sensitive and suitable for the simultaneous determination of glucuronic acid and glucuronolactone.Temperature,pH and solvent affected the stability of glucuronic acid.; 国家海洋公益性行业专项(201005022); 中国大洋矿产资源研究开发协会专项(DY125-15-T-01)资助~~
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/119253]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	范艳群,许建中,徐询,等. 离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性, Simultaneous Determination of Glucuronic Acid and Glucuronolactone Using Ion-exclusion Chromatography[J],2014.
APA	范艳群,许建中,徐询,刘亿婕,&许晨.(2014).离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性..
MLA	范艳群,et al."离子排斥色谱法同时测定葡萄糖醛酸和内酯及葡萄糖醛酸稳定性".(2014).

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