| 高效液相色谱-串联质谱法测定人血浆和尿液中记忆丧失性贝毒软骨藻酸; Determination of Domoic Acid in Human Plasma and Urine by High Performance Liquid Chromatography Tandem Mass Spectrometry | |
| 洪专 ; 张怡评 ; 陈伟珠 ; 高亚辉 | |
| 2014-06-20 | |
| 关键词 | 高效液相色谱-串联质谱法 软骨藻酸 血浆 尿液 HPLC-MS/MS Domoic acid Plasma Urine |
| 英文摘要 | 建立了高效液相色谱-串联三重四极杆质谱法(HPlC-MS/MS)测定人血浆及尿液中记忆丧失性贝毒软骨藻酸。人血浆及尿液样品加入6倍体积甲醇沉淀剂,涡旋离心后,取上清液进样测定。色谱柱为zOrbAX Sb C18柱(150x4.6MM,5μM),柱温30℃;流动相为乙腈-0.1%甲酸水溶液(13∶87,V/V),流速为1.0Ml/MIn。该方法检测血浆和尿液中软骨藻酸的线性范围均为1.8~115μg/l,相关系数r=0.999,检出限为0.9μg/l,定量下限为1.8μg/l。该方法具有操作方便、专属性强、灵敏度高的特点,适用于人体血浆及尿液中软骨藻酸的测定。; High performance liquid chromatography(HPLC)combined with tandem mass spectrometry(LC/MS/MS)has been developed for the determination of domoic acid in human plasma and urine.6 times volume of methanol was added to the sample for protein precipipation;after vortex-centrifuging,the floated supernatant was injected into the HPLC-MS system.The separation was performed on a Zorbax SB C18(150×4.6mm,5μm),using acetonitrile and 0.1% formic acid in water(13∶87,V/V)as the mobile phase with the flowrate of 1.0mL/min.It was found that linear ranges in both plasma and urine samples were 1.8-115μg/L with the correlation coefficients of 0.999.The limit of detection(LOD)was 0.9μg/L and the limit of quantitation(LOQ)was 1.8μg/L.The method is simple,selective and sensitive for detection of domoic acid in human plasma and urine.; 国家973计划(No.2010CB428704); 国家自然科学基金(No.40831160519); 广东海洋经济发展区域示范项目(No.GD2012-D01-001) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/119241]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 洪专,张怡评,陈伟珠,等. 高效液相色谱-串联质谱法测定人血浆和尿液中记忆丧失性贝毒软骨藻酸, Determination of Domoic Acid in Human Plasma and Urine by High Performance Liquid Chromatography Tandem Mass Spectrometry[J],2014. |
| APA | 洪专,张怡评,陈伟珠,&高亚辉.(2014).高效液相色谱-串联质谱法测定人血浆和尿液中记忆丧失性贝毒软骨藻酸.. |
| MLA | 洪专,et al."高效液相色谱-串联质谱法测定人血浆和尿液中记忆丧失性贝毒软骨藻酸".(2014). |
| 个性服务 |
| 查看访问统计 |
| 相关权益政策 |
| 暂无数据 |
| 收藏/分享 |
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。
修改评论