| 沙门氏菌LPS相关抗体的快速检测; A Rapiderodiagnosis For Antibodies Against Salmonella LPS | |
| 宋思扬 ; 郑毅汶 ; 陈晋安 | |
| 1995 | |
| 关键词 | 沙门氏菌 沙门氏菌脂多糖 乳胶颗粒凝集 乳胶－磁性颗粒检测 Salmonella Lipopolysaccharide of salmonella. Latex agglutination La-tex-magnetic polystyrene beads assay |
| 英文摘要 | 建立两种颗粒——乳胶颗粒及磁性颗粒快速检测沙门氏菌lPS相关抗体（IgM或Igg的方法。乳胶颗粒用lPS包被，磁性颗粒用羊抗人IgM或Igg包被，将两种颗粒及血清标本置于酶标条的微孔中，室温振荡30MIn后置于磁铁上使磁性颗粒沉淀。上清液在400nM的酶标仪上读取浊度以判定反应结果。该方法比乳胶凝集试验的灵敏度高。其结果与用ElISA方法检测的结果一致，但步骤少，时间短，操作更简便。是一种快速诊断沙门氏菌感染的有效方法。; An eFFicient assay has been developed For detection antibodies againstSalmonella lipopolysaccharide(LPS).Latex coated with I/PS,polystyrene beads( Dynabeads )coated with goat anti-human μ or γ,and serum is mixed in the well of micro plate and shaken For30 min at room temperature。Then sediment by a magnet, the turbidity of the resuItant latexsuspension is measured spectrophotometrically at wavelength of 400 nm.The assay gives a highersensitivity as compared with latex agglutination.The results are in accordance with ELISA,while easier and can be accomplished by Fewer steps. |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/119054]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 宋思扬,郑毅汶,陈晋安. 沙门氏菌LPS相关抗体的快速检测, A Rapiderodiagnosis For Antibodies Against Salmonella LPS[J],1995. |
| APA | 宋思扬,郑毅汶,&陈晋安.(1995).沙门氏菌LPS相关抗体的快速检测.. |
| MLA | 宋思扬,et al."沙门氏菌LPS相关抗体的快速检测".(1995). |
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