| 人角质细胞生长因子-1在水稻中的表达; Recombinant Keratinocyte Growth Factor-1 Expression in Rice | |
| 张国广 ; 苏勇波 ; 沈明山 ; 陈亮 | |
| 2010 | |
| 关键词 | 角质细胞生长因子 水稻遗传转化 基因表达 keratinocyte growth factor-1 rice genetic transformation gene expression |
| 英文摘要 | 人角质细胞生长因子(kErATInOCyTE grOWTH fACTOr,kgf)在组织的损伤修复中起着重要的作用.植物细胞生物反应器是一种成本低、安全性好的蛋白生产系统.本研究将人kgf-1经农杆菌介导法转入水稻中,共获得38株抗性再生植株.SOuTHErn杂交和rT-PCr结果表明kgf-1基因整合入水稻基因组中且部分转化株中kgf-1基因得到转录.WESTErn blOTTIng检测结果显示有两株转化水稻中能够表达kgf-1.; Keratinocyte growth factor(KGF) plays an important role in the tissue damage repair.Plant cell bioreactor is a low cost and safe protein production system.Human KGF-1 gene was transformed into rice by Agrobacterium-mediated method and a total of 38 putative transgenic rice plants were obainted.The results of southern blotting and RT-PCR assay showed that KGF-1 gene was integrated into the rice genome and KGF-1 gene was transcribed in part of the putative transformed rices.Western blotting results showed that KGF-1 was expressed in two transgenic rices.; 福建省自然科学基金资助项目(2010J01240) |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/119011]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 张国广,苏勇波,沈明山,等. 人角质细胞生长因子-1在水稻中的表达, Recombinant Keratinocyte Growth Factor-1 Expression in Rice[J],2010. |
| APA | 张国广,苏勇波,沈明山,&陈亮.(2010).人角质细胞生长因子-1在水稻中的表达.. |
| MLA | 张国广,et al."人角质细胞生长因子-1在水稻中的表达".(2010). |
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