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	维甲酸对人神经母细胞瘤SK-N-SH细胞形态结构和分化标志物表达的影响; The Effects of Retinoic Acid on the Morphology and Terminal Differentiation of SK-N-SH Cell
	张秀艳 ; 梁盈 ; 李祺福 ; 赵振利 ; 荆光军
	2009
关键词	维甲酸 细胞分化 神经母细胞瘤细胞 细胞形态与超微结构 终末分化指标 Retinoic Acid Cell differentiation Neurblastoma Cell morphology and ultrastructure Index of terminal differentiation
英文摘要	目的:观察维甲酸对人神经母细胞瘤Sk-n-SH细胞形态与超微结构及其相关标志物表达的影响,以鉴定其对神经母细胞瘤细胞终末分化的诱导作用。方法:1μMOl/l维甲酸处理Sk-n-SH细胞,光镜、电镜和免疫细胞化学检测研究Sk-n-SH细胞处理前后细胞形态、超微结构变化和神经元相关标志物的表达变化。结果:光镜与电镜观察结果显示,Sk-n-SH细胞经1μMOl/lrA处理后,细胞形态和超微结构产生了细胞呈极性状、伸出多个轴突树突状突起、细胞逐渐变小变圆并融合在一起形成类似神经节样结构、细胞表面微绒毛减少、核仁变少变小、常染色质增多、细胞器丰富发达等显著变化;免疫细胞化学检测显示经rA处理后Sk-n-SH细胞nSE、MAP2、SynAPTOPHySIn的表达较对照组细胞明显加强。结论:维甲酸能改变Sk-n-SH细胞形态和超微结构恶性表型特征,并促进与神经细胞相关的终末分化指标的表达,从而对人神经母细胞瘤细胞的终未分化具有显著的诱导作用。; Objective:To investigate the biological effects of retinoic acid on the cell morphology and terminal differentiation of the human neuroblastoma SK-N-SH cells.Methods:After treated with 1μmol/L retinoic acid,SK-N-SH cells were subjected to light-microscopy, electro-microscopy and immuncocytochemical assay,and were compared with the untreated cell.Results:The results of light and electro-microscopy observation showed that the morphology and ultrastructure of SK-N-SH cells had changed after treatment.Cells became multipolar with several neuritis;cell bodies became smaller with long axonal processes between one another to form ganglion; less microvilli in the cell faces;the nucleolus reduced while the euchromatin increased;and the organelles developed.Immuncocytochemical analysis revealed that the expression of the neuronal markers of terminal differentiation,including NSE,MAP2 and Synaptophysin, were strongly positive after treatment.Conclusions:Retinoic acid could reverse the malignant phenotypes of SK-N-SH cells and promote the expression of terminal differentiation biomarkers,thus playing a significant role in cell inducement into terminal differentiation.; 国家自然科学基金(No.30470877)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/118941]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	张秀艳,梁盈,李祺福,等. 维甲酸对人神经母细胞瘤SK-N-SH细胞形态结构和分化标志物表达的影响, The Effects of Retinoic Acid on the Morphology and Terminal Differentiation of SK-N-SH Cell[J],2009.
APA	张秀艳,梁盈,李祺福,赵振利,&荆光军.(2009).维甲酸对人神经母细胞瘤SK-N-SH细胞形态结构和分化标志物表达的影响..
MLA	张秀艳,et al."维甲酸对人神经母细胞瘤SK-N-SH细胞形态结构和分化标志物表达的影响".(2009).

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