| 酵母双杂交技术筛选与Axin相互作用的蛋白质; Screening of Axin-interacting Proteins by Yeast Two-hybrid System | |
| 叶志云 ; 张婷婷 ; 贺颖 ; 陈巧玉 ; 刘琪 | |
| 2009 | |
| 关键词 | Axin 酵母双杂交 相互作用 Axin yeast two-hybrid interaction |
| 英文摘要 | 肿瘤抑制因子AXIn以构架蛋白的形式通过WnT、Jnk、P53、Tgf-β、g蛋白等众多信号转导途径参与细胞生长、增殖、分化和凋亡等多种重要细胞命运的调控过程.为了发现AXIn新的相互作用蛋白,进一步研究AXIn的多功能性,作者通过酵母双杂交技术,将AXIn1基因从SPEI单一酶切位点到3′末端的dnA序列构建到PgbkT7载体作为诱饵,对小鼠脑的CdnA文库进行筛选,最后获得28个阳性克隆.通过对这些克隆的测序和对所得序列的生物信息学分析,得到13个与AXIn的C末端相互作用的蛋白质,这些蛋白主要参与胚胎发育,肿瘤形成,蛋白翻译后修饰等生物学过程的调节.这一结果为进一步研究AXIn的新功能提供了很好的线索.; The tumor suppressor Axin regulates cell growth,proliferation and axis formation by participating in many important signal transduction pathways including Wnt,JNK,p53,TGF-β and G-protein pathways as a scaffold protein.In this study,we carried out a yeast two-hybrid screen for proteins that interact with Axin.The DNA fragment from the Spe I site to 3′-end of mouse Axin1 gene coding the C-terminal region of Axin was subcloned into pGBKT7 vector(SpeI-CT-BD),and the mouse brain cDNA library was screened by this bait.We obtained 28 positive clones,13 of which carried in-frame protein-coding sequences.These new interaction partners mainly related to embryo development,tumorigenesis,and protein modification.These results provided an important insight for further studying new functions of Axin,a multifunctional scaffold protein.; 国家基础学科人才培养基金(J0630649);福建省自然科学基金(2008J0109)资助 |
| 语种 | zh_CN |
| 内容类型 | 期刊论文 |
| 源URL | [http://dspace.xmu.edu.cn/handle/2288/118898]  |
| 专题 | 生命科学－已发表论文 |
| 推荐引用方式 GB/T 7714 | 叶志云,张婷婷,贺颖,等. 酵母双杂交技术筛选与Axin相互作用的蛋白质, Screening of Axin-interacting Proteins by Yeast Two-hybrid System[J],2009. |
| APA | 叶志云,张婷婷,贺颖,陈巧玉,&刘琪.(2009).酵母双杂交技术筛选与Axin相互作用的蛋白质.. |
| MLA | 叶志云,et al."酵母双杂交技术筛选与Axin相互作用的蛋白质".(2009). |
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