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	虹鳟腺苷酸转移酶基因2(ant2)cDNA克隆与蛋白结构分析; Cloning and Analysis of ant2 Full-Length cDNA Derived from Rainbow Trout(Oncorhynchus mykiss)
	王家庆 ; 刘晓慧 ; 李代宗 ; 宋波澜 ; 王志平 ; 郭冉
	2009
关键词	虹鳟鱼 腺苷酸转移酶基因2 RT-PCR cDNA末端快速扩增 rainbow trout(Oncorhynchus mykiss) ant2 RT-PCR rapid amplification of cDNA ends(RACE)
英文摘要	腺苷酸转移酶(AnT)是位于线粒体内膜上参与能量分子传导的转运蛋白,在细胞凋亡调控网络中发挥重要作用.以虹鳟鱼(OnCOrHynCHuS MykISS)为研究材料,使用rT-PCr和rACE法分离和克隆了虹鳟鱼AnT2基因CdnA的全长序列(gEnbAnk登录号:fJ591153).该序列全长1 276 bP,其中5′端非翻译区长66 bP,3′端非翻译区长316 bP,开放性阅读框长894 bP,编码297个氨基酸.该蛋白具有3个保守的线粒体穿膜功能结构域和3个转膜区.通过与其他脊椎动物腺苷酸转移酶蛋白序列的比较,发现该基因具有高度保守性,氨基酸序列的同源性均在90%左右.同源模建显示其与牛的线粒体AdP/ATP载体,CHAIn A蛋白有相同的孔洞(CAVITy)结构.; The adenine nucleotide translocase(ANT) family of proteins are the most abundant inner membrane proteins of the mitochondrion.They are responsible for the transport of adenine nucleotides across the inner mitochondrial membrane,importing ADP for oxidative phosphorylation and exporting ATP to the cytosol.In this paper,RT-PCR and rapid amplification of cDNA ends(RACE) methods were used for the isolation of the whole cDNA of ant2 gene(GenBank accession number: FJ591153) from rainbow trout Oncorhynchus mykiss.Sequence analysis reveals a 1 276 bp cDNA containing 66 bp 5′-UTR,316 bp 3′-UTR and 894 bp ORF encoding 297 amino acids.It also has three conserved domains of mitochondrial carrier proteins and three transmembrane helixes.ANT2 shows extensive similarities to the known ADP/ATP translocase polypeptides,and the similarity is about 90% between vertebrates.Homology modeling shows that rainbow trout ANT2 protein has the same cavity structure with the ADP/ATP carrier,chain A of cattle.; 河北省科技厅科技计划项目(0624240011)
语种	zh_CN
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/118886]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	王家庆,刘晓慧,李代宗,等. 虹鳟腺苷酸转移酶基因2(ant2)cDNA克隆与蛋白结构分析, Cloning and Analysis of ant2 Full-Length cDNA Derived from Rainbow Trout(Oncorhynchus mykiss)[J],2009.
APA	王家庆,刘晓慧,李代宗,宋波澜,王志平,&郭冉.(2009).虹鳟腺苷酸转移酶基因2(ant2)cDNA克隆与蛋白结构分析..
MLA	王家庆,et al."虹鳟腺苷酸转移酶基因2(ant2)cDNA克隆与蛋白结构分析".(2009).

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