Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L

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	Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L
	Pan, Zhizhen ; Xu, Lian ; Zhu, Yujing ; Shi, Huai ; Chen, Zheng ; Chen, Meichun ; Chen, Qingxi ; Liu, Bo ; Zhu YJ(朱育菁) ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1007/s11274-014-1689-x
	2014
关键词	Agriculture Amino acids Bacteriology Bioassay Genes
英文摘要	Bacillus thuringiensis (Bt) strain FJAT-12 was a novel Bt strain isolated by Agricultural Bio-Resources Institute, Fujian Academy of Agricultural Science. In this study, a new cry2Ab gene was cloned from Bt strain FJAT-12 and named as cry2Ab30 by Bt delta-endotoxin Nomenclature Committee. The sequencing results showed there were two mutations in conservative sites which led to two amino acids modification. Homology modeling indicated that the two changes were located in β-sheet of Domain II. A prokaryotic expression vector pET30a-cry2Ab30 was constructed and the expressed protein was analyzed by western blot using Cry2Ab antibody. The expression conditions including IPTG concentration, revolution and temperature were optimized to get the highest expression level by SDS-PAGE and BandScan. The bioassay results also showed that the Cry2Ab30 toxin had high insecticidal activity against Plutella xylostella and the LC50 value was 0.0103 μg.mL-1. The two mutations in β-sheet of Domain II might contribute to insecticidal activity of Cry2Ab30 toxin against Plutella xylostella. ? 2014 Springer Science+Business Media Dordrecht.
语种	英语
出版者	Springer Science+Business Media Dordrecht.
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90903]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Pan, Zhizhen,Xu, Lian,Zhu, Yujing,et al. Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L[J]. http://dx.doi.org/10.1007/s11274-014-1689-x,2014.
APA	Pan, Zhizhen.,Xu, Lian.,Zhu, Yujing.,Shi, Huai.,Chen, Zheng.,...&陈清西.(2014).Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L.http://dx.doi.org/10.1007/s11274-014-1689-x.
MLA	Pan, Zhizhen,et al."Characterization of a new cry2Ab gene of Bacillus thuringiensis with high insecticidal activity against Plutella xylostella L".http://dx.doi.org/10.1007/s11274-014-1689-x (2014).