Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense

CORC > 厦门大学 > 生命科学－已发表论文

	Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense
	Yang, Xiao ; Li, Xinyi ; Zhou, Yanyan ; Zheng, Wei ; Yu, Changping ; Zheng, Tianling ; Zheng TL(郑天凌)
刊名	http://dx.doi.org/10.1016/j.scitotenv.2014.02.125
	2014
关键词	Bioassay Cell death Nucleic acids Oxygen
英文摘要	Algicidal bacteria may play a major role in controlling harmful algal blooms (HABs) dynamics. Bacterium DH77-1 was isolated with high algicidal activity against the toxic dinoflagellate Alexandrium tamarense and identified as Joostella sp. DH77-1. The results showed that DH77-1 exhibited algicidal activity through indirect attack, which excreted active substance into the filtrate. It had a relatively wide host range and the active substance of DH77-1 was relatively stable since temperature, pH and storage condition had no obvious effect on the algicidal activity. The algicidal compound from bacterium DH77-1 was isolated based on activity-guided bioassay and the molecular weight was determined to be 125.88 by MALDI-TOF mass spectrometer, however further identification via nuclear magnetic resonance (NMR) spectra is ongoing. The physiological responses of algal cells after exposure to the DH77-1 algicidal substances were as follows: the antioxidant system of A. tamarense responded positively in self-defense; total protein content decreased significantly as did the photosynthetic pigment content; superoxide dismutase, peroxidase enzyme and malondialdehyde content increased extraordinarily and algal cell nucleic acid leaked seriously ultimately inducing cell death. Furthermore, DH77-1 is the first record of a Joostella sp. bacterium being algicidal to the harmful dinoflagellate A. tamarense, and the bacterial culture and the active compounds might be potentially used as a bio-agent for controlling harmful algal blooms. ? 2014 Elsevier B.V.
语种	英语
出版者	ELSEVIER SCIENCE BV
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90896]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Yang, Xiao,Li, Xinyi,Zhou, Yanyan,et al. Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense[J]. http://dx.doi.org/10.1016/j.scitotenv.2014.02.125,2014.
APA	Yang, Xiao.,Li, Xinyi.,Zhou, Yanyan.,Zheng, Wei.,Yu, Changping.,...&郑天凌.(2014).Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense.http://dx.doi.org/10.1016/j.scitotenv.2014.02.125.
MLA	Yang, Xiao,et al."Novel insights into the algicidal bacterium DH77-1 killing the toxic dinoflagellate Alexandrium tamarense".http://dx.doi.org/10.1016/j.scitotenv.2014.02.125 (2014).