Point-of-care test for C-reactive protein by a fluorescence-based lateral flow immunoassay

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	Point-of-care test for C-reactive protein by a fluorescence-based lateral flow immunoassay
	Gu, Yongbin ; Yang, Yongliang ; Zhang, Jing ; Ge, ShengXiang ; Tang, Zhanghong ; Qiu, Xianbo ; Ge SX(葛胜祥) ; Qiu XB(邱宪波)
刊名	http://dx.doi.org/10.1080/10739149.2014.930877
	2014
关键词	Amplifiers (electronic) Calibration Fluorescence Immunology Light emitting diodes
英文摘要	An innovative, portable fluorescence reader was developed for the determination of C-reactive protein based on a lateral flow immunoassay. The C-reactive protein concentration was proportional to the intensity of the test line which was calibrated relative to the control line. To quantify the fluorescence intensity of the lateral flow strip, a custom illumination module, which concentrated the excitation beam from an ultraviolet light-emitting diode, was developed for strip scanning. Accordingly, a high sensitive photodiode with a preamplifier was chosen as the detector for fluorescence. For good repeatability, the strip scanning resolution was set to 5 μm between data points by controlling a linear stage actuated by a stepper motor. Four double-logistic calibration models were compared. The sensitivity for C-reactive protein was 0.1 mg/L and the linear dynamic range extended to 400 mg/L. The optical reader provides a new and simple approach for the determination of C-reactive protein and may be modified for other similar biomarkers.
语种	英语
出版者	Taylor and Francis Inc.
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90875]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Gu, Yongbin,Yang, Yongliang,Zhang, Jing,et al. Point-of-care test for C-reactive protein by a fluorescence-based lateral flow immunoassay[J]. http://dx.doi.org/10.1080/10739149.2014.930877,2014.
APA	Gu, Yongbin.,Yang, Yongliang.,Zhang, Jing.,Ge, ShengXiang.,Tang, Zhanghong.,...&邱宪波.(2014).Point-of-care test for C-reactive protein by a fluorescence-based lateral flow immunoassay.http://dx.doi.org/10.1080/10739149.2014.930877.
MLA	Gu, Yongbin,et al."Point-of-care test for C-reactive protein by a fluorescence-based lateral flow immunoassay".http://dx.doi.org/10.1080/10739149.2014.930877 (2014).