CORC  > 厦门大学  > 生命科学-已发表论文
Protection against Lethal Enterovirus 71 Challenge in Mice by a Recombinant Vaccine Candidate Containing a Broadly Cross-Neutralizing Epitope within the VP2 EF Loop
Xu, Longfa ; He, Delei ; Li, Zhiqun ; Zheng, Jun ; Yang, Lisheng ; Yu, Miao ; Yu, Hai ; Chen, Yixin ; Que, Yuqiong ; Shih, James Wai Kuo ; Liu, Gang ; Zhang, Jun ; Zhao, Qinjian ; Cheng, Tong ; Xia, Ning-shao ; Chen YX(陈毅歆) ; Liu G(刘刚) ; Zhang J(张军) ; Zhao QJ(赵勤俭) ; Xia NS(夏宁邵)
刊名http://dx.doi.org/10.7150/thno.7457
2014
关键词HEPATITIS-B-VIRUS SELECTIN GLYCOPROTEIN LIGAND-1 CAPSID PROTEIN MOUTH-DISEASE EXTERNALIZED POLYPEPTIDE 3-DIMENSIONAL STRUCTURE MONOCLONAL-ANTIBODIES COXSACKIEVIRUS A21 CELLULAR RECEPTOR CRYSTAL-STRUCTURE
英文摘要National Science and Technology Major Project of the Ministry of Science and Technology of China [2010ZX09401-403]; National Natural Science Foundation of China [30972826]; National Natural Science Fund for Distinguished Young Scholar [30925030]; Human enterovirus 71 (EV71) is the main causative agent of hand, foot, and mouth disease (HFMD) and is associated with several severe neurological complications in the Asia-Pacific region. Here, we evaluated that while passive transfer of neutralizing monoclonal antibody (nMAb) against the VP2 protein protect against lethal EV71 infection in BALB/c mice. Protective nMAb were mapped to residues 141-155 of VP2 by peptide ELISA. High-resolution structural analysis showed that the epitope is part of the VP2 EF loop, which is the "puff" region that forms the "southern rim" of the canyon. Moreover, a three-dimensional structural characterization for the puff region with prior neutralizing epitopes and receptor-binding sites that can serve to inform vaccine strategies. Interestingly, using hepatitis B virus core protein (HBc) as a carrier, we demonstrated that the cross-neutralizing EV71 antibodies were induced, and the VP2 epitope immunized mice serum also conferred 100% in vivo passive protection. The mechanism of in vivo protection conferred by VP2 nMAb is in part attributed to the in vitro neutralizing titer and ability to bind authentic viral particles. Importantly, the anti-VP2(aa141-155) antibodies could inhibit the binding of human serum to EV71 virions showed that the VP2 epitope is immunodominant. Collectively, our results suggest that a broad-spectrum vaccine strategy targeting the high-affinity epitope of VP2 EF loop may elicits effective immune responses against EV71 infection.
语种英语
出版者IVYSPRING INT PUBL
内容类型期刊论文
源URL[http://dspace.xmu.edu.cn/handle/2288/90852]  
专题生命科学-已发表论文
推荐引用方式
GB/T 7714
Xu, Longfa,He, Delei,Li, Zhiqun,et al. Protection against Lethal Enterovirus 71 Challenge in Mice by a Recombinant Vaccine Candidate Containing a Broadly Cross-Neutralizing Epitope within the VP2 EF Loop[J]. http://dx.doi.org/10.7150/thno.7457,2014.
APA Xu, Longfa.,He, Delei.,Li, Zhiqun.,Zheng, Jun.,Yang, Lisheng.,...&夏宁邵.(2014).Protection against Lethal Enterovirus 71 Challenge in Mice by a Recombinant Vaccine Candidate Containing a Broadly Cross-Neutralizing Epitope within the VP2 EF Loop.http://dx.doi.org/10.7150/thno.7457.
MLA Xu, Longfa,et al."Protection against Lethal Enterovirus 71 Challenge in Mice by a Recombinant Vaccine Candidate Containing a Broadly Cross-Neutralizing Epitope within the VP2 EF Loop".http://dx.doi.org/10.7150/thno.7457 (2014).
个性服务
查看访问统计
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。


©版权所有 ©2017 CSpace - Powered by CSpace