The p53-induced factor Ei24 inhibits nuclear import through an importin beta-binding-like domain

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	The p53-induced factor Ei24 inhibits nuclear import through an importin beta-binding-like domain
	Lieu, Kim G. ; Shim, Eun-Hee ; Wang, Jinling ; Lokareddy, Ravi K. ; Tao, Tao ; Cingolani, Gino ; Zambetti, Gerard P. ; Jans, David A. ; Tao T(陶涛)
刊名	http://dx.doi.org/10.1083/jcb.201304055
	2014-05-12
关键词	LOCALIZATION SIGNAL ENDOPLASMIC-RETICULUM BREAST-CANCER NEGATIVE REGULATOR TRANSPORT RECEPTOR TERMINAL DOMAIN PROTEIN IMPORT ALPHA P53 IDENTIFICATION
英文摘要	National Health and Medical Research Council Australia [APP 1002486, 491055]; National Institutes of Health/National Cancer Institute Cancer Center Support CORE grant [CA21765]; American Lebanese Syrian Associated Charities (ALSAC) of St. Jude Children's Research Hospital; Ministry of Science and Technology, China [2013CB910803]; The etoposide-induced protein E124 was initially identified as a p53-responsive, proapoptotic factor, but no clear function has been described. Here, we use a nonbiased. proteomics approach to identify members of the importin (IMP) family of nuclear transporters as interactors of Ei24 and characterize an IMP beta-binding-like (IBBL) domain within Ei24. We show that Ei24 can bind specifically to IMP beta 1 and IMP alpha 2, but not other IMPs, and use a mutated IMP beta l derivative to show that Ei24 binds to the same site on IMP beta 1 as the IMP alpha. IBB. Ectopic expression of Ei24 reduced the extent of IMPplor IMP alpha/beta 1-dependent nuclear protein import specifically, whereas specific alanine substitutions within the IBBL abrogated this activity. Induction of endogenous Ei24 expression through etoposide treatment similarly inhibited nuclear import in a mouse embryonic fibroblast model. Thus, Ei24 can bind specifically to IMP beta 1 and IMP alpha 2 to impede their normal role in nuclear import, shedding new light on the cellular functions of EN and its tumor suppressor role.
语种	英语
出版者	ROCKEFELLER UNIV PRESS
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90781]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Lieu, Kim G.,Shim, Eun-Hee,Wang, Jinling,et al. The p53-induced factor Ei24 inhibits nuclear import through an importin beta-binding-like domain[J]. http://dx.doi.org/10.1083/jcb.201304055,2014.
APA	Lieu, Kim G..,Shim, Eun-Hee.,Wang, Jinling.,Lokareddy, Ravi K..,Tao, Tao.,...&陶涛.(2014).The p53-induced factor Ei24 inhibits nuclear import through an importin beta-binding-like domain.http://dx.doi.org/10.1083/jcb.201304055.
MLA	Lieu, Kim G.,et al."The p53-induced factor Ei24 inhibits nuclear import through an importin beta-binding-like domain".http://dx.doi.org/10.1083/jcb.201304055 (2014).