Identification of a Highly Conserved and Surface Exposed B-cell Epitope on the Nucleoprotein of Influenza A Virus

CORC > 厦门大学 > 生命科学－已发表论文

	Identification of a Highly Conserved and Surface Exposed B-cell Epitope on the Nucleoprotein of Influenza A Virus
	Gui, Xun ; Ge, Pinghui ; Wang, Xuliang ; Yang, Kunyu ; Yu, Hai ; Zhao, Qinjian ; Chen, Yixin ; Xia, Ningshao ; Yang KY(杨坤宇) ; Yu H(俞海) ; Zhao QJ(赵勤俭) ; Chen YX(陈毅歆) ; Xia NS(夏宁邵)
刊名	http://dx.doi.org/10.1002/jmv.23812
	2014
关键词	OSELTAMIVIR-RESISTANT INFLUENZA H7N9 VIRUS RIBONUCLEOPROTEIN COMPLEXES HUMAN INFECTION RECOGNITION H3N2 RNA
英文摘要	National Natural Science Foundation of China [81202255]; Scientific and Technological Major Special Project of China [2011ZX09102-009-12]; Hong Kong, Macao, and Taiwan Science and Technology Cooperation Special Project of China [2012DFH30020]; Influenza virus still poses a major threat to human health worldwide. The nucleoprotein (NP) of influenza A virus plays an essential role in the viral replication and transcription and hence becomes a promising therapeutic target. NP forms a complicated conformation under native conditions and might denature when performing immunoassays such as western blot in the study of NP function. Therefore, it is useful to make an NP specific monoclonal antibody (mAb) that recognizes linear epitope instead of conformational epitope. In this study, a recombinant NP (rNP) of influenza A virus was over-expressed and used to generate a panel of anti-NP mAbs. These anti-NP mAbs were grouped into three classes based on their reactivity in Western blots. Only Class I mAb can react with linear rNP fragments. One of Class I mAb, 4D2, was characterized further by epitope mapping with a series of overlapping synthetic peptides, indicating that the 4D2 epitope is a surface exposed, linear epitope between amino acid residues 243 and 251. This epitope is highly conserved among different influenza A viruses with an identity of 98.4% (17,922/18,210). Western blot, co-immunoprecipitation, immunofluorescence, and immunohistochemistry experiments all indicated 4D2 is highly specific to NP of influenza A virus. The results demonstrated that 4D2 can be used as a research tool for functional study of NP in the replication cycle of influenza A virus. Further work is needed to understand the function and importance of this epitope. J. Med. Virol. 86:995-1002, 2014. (c) 2013 Wiley Periodicals, Inc.
语种	英语
出版者	WILEY-BLACKWELL
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90775]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Gui, Xun,Ge, Pinghui,Wang, Xuliang,et al. Identification of a Highly Conserved and Surface Exposed B-cell Epitope on the Nucleoprotein of Influenza A Virus[J]. http://dx.doi.org/10.1002/jmv.23812,2014.
APA	Gui, Xun.,Ge, Pinghui.,Wang, Xuliang.,Yang, Kunyu.,Yu, Hai.,...&夏宁邵.(2014).Identification of a Highly Conserved and Surface Exposed B-cell Epitope on the Nucleoprotein of Influenza A Virus.http://dx.doi.org/10.1002/jmv.23812.
MLA	Gui, Xun,et al."Identification of a Highly Conserved and Surface Exposed B-cell Epitope on the Nucleoprotein of Influenza A Virus".http://dx.doi.org/10.1002/jmv.23812 (2014).