Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity

CORC > 厦门大学 > 生命科学－已发表论文

	Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity
	Huang, Lixing ; Zuo, Zhenghong ; Zhang, Youyu ; Wu, Meifang ; Lin, Jackie Jin ; Wang, Chonggang ; Zuo ZH(左正宏) ; Wang ZG(王重刚)
刊名	http://dx.doi.org/10.1016/j.chemosphere.2014.02.078
	2014
关键词	POLYCYCLIC AROMATIC-HYDROCARBONS SEBASTISCUS-MARMORATUS EMBRYOS REAL-TIME PCR CONGENITAL-ANOMALIES EXPOSURE INFLUENCES GENE-EXPRESSION DNA-ADDUCTS DEFECTS BENZO PYRENE POLLUTION
英文摘要	National Natural Science Foundation of China [21177103]; Fundamental Research Funds for the Central Universities [2012121045]; Benzo(a)pyrene (BaP) is a representative polycyclic aromatic hydrocarbon (PAH), which is ubiquitous in the environment. The toxic effects of BaP on fish embryos have been described in detail, but some potentially toxic effects of BaP might have been neglected owing to the limitations of traditional techniques. In the present research, global transcriptional patterns were used to study the potentially toxic effects of BaP, as well as its underlying toxicological mechanisms. The expression levels of multiple genes were significantly changed by BaP exposure. The results of ontology assignments and cluster analysis showed that BaP could affect the processes of photoreceptor maintenance and phototransduction. We also conducted an experiment on phototactic response and found that larvae exposed to BaP displayed a decreasing response to light. In addition, BaP exposure decreased the cellular density of the ganglion cell layer (GCL) significantly. These results suggested that BaP exposure induced visual system developmental defects and dysfunction by perturbation of photoreceptor development related genes. Our results were helpful for an understanding of the toxicity of BaP. This study also indicated that microarray analysis was effective for predicting the potential toxicity of chemicals with high sensitivity and accuracy. (C) 2014 Elsevier Ltd. All rights reserved.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90755]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Huang, Lixing,Zuo, Zhenghong,Zhang, Youyu,et al. Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity[J]. http://dx.doi.org/10.1016/j.chemosphere.2014.02.078,2014.
APA	Huang, Lixing.,Zuo, Zhenghong.,Zhang, Youyu.,Wu, Meifang.,Lin, Jackie Jin.,...&王重刚.(2014).Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity.http://dx.doi.org/10.1016/j.chemosphere.2014.02.078.
MLA	Huang, Lixing,et al."Use of toxicogenomics to predict the potential toxic effect of Benzo(a)pyrene on zebrafish embryos: Ocular developmental toxicity".http://dx.doi.org/10.1016/j.chemosphere.2014.02.078 (2014).