Crosstalk between casein kinase II and Ste20-related kinase Nak1

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	Crosstalk between casein kinase II and Ste20-related kinase Nak1
	Cipak, Lubos ; Gupta, Sneha ; Rajovic, Iva ; Jin, Quan-Wen ; Anrather, Dorothea ; Ammerer, Gustav ; McCollum, Dannel ; Gregan, Juraj ; Jin QW(靳全文)
刊名	http://dx.doi.org/10.4161/cc.24095
	2013
关键词	FISSION YEAST PROTEIN-KINASE SCHIZOSACCHAROMYCES-POMBE CELL MORPHOGENESIS BETA-SUBUNIT MEIOSIS I CK2 CYCLE REGULATORS LANDSCAPE
英文摘要	Austrian Science Fund [P23609, P21437, F3403]; HFSP [RGY0069/2010]; Slovak Research and Development Agency [APVV-0111-12, APVV-0334-12]; European Community [PERG07-GA-2010-268167, PCIG11-GA-2012-322300]; National Institutes of Health [GM058406-14]; Although the sterile 20 (Ste20) serine/threonine protein kinase was originally identified as a component of the S. cerevisiae mating pathway, it has homologs in higher eukaryotes and is part of a larger family of Ste20-like kinases. Ste20-like kinases are involved in multiple cellular processes, such as cell growth, morphogenesis, apoptosis and immune response. Carrying out such a diverse array of biological functions requires numerous regulatory inputs and outputs in the form of protein-protein interactions and post-translational modifications. Hence, a thorough knowledge of Ste20-like kinase binding partners and phosphorylation sites will be essential for understanding the various roles of these kinases. Our recent study revealed that Schizosaccharomyces pombe Nak1 (a conserved member of the GC-kinase sub-family of Ste20-like kinases) is in a complex with the leucine-rich repeat-containing protein Sog2. Here, we show a novel and unexpected interaction between the Nak1-Sog2 kinase complex and Casein kinase 2 (Cka1, Ckb1 and Ckb2) using tandem-affinity purification followed by mass spectrometric analysis. In addition, we identify unique phosphosites on Nak1, Sog2 and the catalytic subunit of casein kinase 2, Cka1. Given the conserved nature of these kinases, we expect this work will shed light on the functions of these proteins both in yeast and higher eukaryotes.
语种	英语
出版者	LANDES BIOSCIENCE
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90743]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Cipak, Lubos,Gupta, Sneha,Rajovic, Iva,et al. Crosstalk between casein kinase II and Ste20-related kinase Nak1[J]. http://dx.doi.org/10.4161/cc.24095,2013.
APA	Cipak, Lubos.,Gupta, Sneha.,Rajovic, Iva.,Jin, Quan-Wen.,Anrather, Dorothea.,...&靳全文.(2013).Crosstalk between casein kinase II and Ste20-related kinase Nak1.http://dx.doi.org/10.4161/cc.24095.
MLA	Cipak, Lubos,et al."Crosstalk between casein kinase II and Ste20-related kinase Nak1".http://dx.doi.org/10.4161/cc.24095 (2013).