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	Divalent cation tolerance protein binds to beta-secretase and inhibits the processing of amyloid precursor protein
	Liu, Runzhong ; Hou, Haibo ; Yi, Xuelian ; Wu, Shanwen ; Zeng, Huan ; Liu RZ(刘润忠)
刊名	http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.004
	2013
关键词	GATED SODIUM-CHANNELS ALZHEIMERS-DISEASE PEPTIDE GENERATION TRANSGENIC MICE MEMORY DEFICITS GAMMA-SECRETASE BACE1 ACETYLCHOLINESTERASE CLEAVAGE BRAIN
英文摘要	National Natural Science Foundation of China [81171192]; XMU Basic Training Program of Undergraduate [CXB2011019]; Key Laboratory of Ministry of Education for Cell Biology and Tumor Cell Engineering of Xiamen University [201101]; The deposition of amyloid-beta is a pathological hallmark of Alzheimer's disease. Amyloid-beta is derived from amyloid precursor protein through sequential proteolytic cleavages by beta-secretase (beta-site amyloid precursor protein-cleaving enzyme 1) and gamma-secretase. To further elucidate the roles of beta-site amyloid precursor protein-cleaving enzyme 1 in the development of Alzheimer's disease, a yeast two-hybrid system was used to screen a human embryonic brain cDNA library for proteins directly interacting with the intracellular domain of beta-site amyloid precursor protein-cleaving enzyme 1. A potential beta-site amyloid precursor protein-cleaving enzyme 1-interacting protein identified from the positive clones was divalent cation tolerance protein. Immunoprecipitation studies in the neuroblastoma cell line N2a showed that exogenous divalent cation tolerance protein interacts with endogenous beta-site amyloid precursor protein-cleaving enzyme 1. The overexpression of divalent cation tolerance protein did not affect beta-site amyloid precursor protein-cleaving enzyme 1 protein levels, but led to increased amyloid precursor protein levels in N2a/APP695 cells, with a concomitant reduction in the processing product amyloid precursor protein C-terminal fragment, indicating that divalent cation tolerance protein inhibits the processing of amyloid precursor protein. Our experimental findings suggest that divalent cation tolerance protein negatively regulates the function of beta-site amyloid precursor protein-cleaving enzyme 1. Thus, divalent cation tolerance protein could play a protective role in Alzheimer's disease.
语种	英语
出版者	SHENYANG EDITORIAL DEPT NEURAL REGENERATION RES
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90733]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liu, Runzhong,Hou, Haibo,Yi, Xuelian,et al. Divalent cation tolerance protein binds to beta-secretase and inhibits the processing of amyloid precursor protein[J]. http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.004,2013.
APA	Liu, Runzhong,Hou, Haibo,Yi, Xuelian,Wu, Shanwen,Zeng, Huan,&刘润忠.(2013).Divalent cation tolerance protein binds to beta-secretase and inhibits the processing of amyloid precursor protein.http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.004.
MLA	Liu, Runzhong,et al."Divalent cation tolerance protein binds to beta-secretase and inhibits the processing of amyloid precursor protein".http://dx.doi.org/10.3969/j.issn.1673-5374.2013.11.004 (2013).

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