The orphan nuclear receptor TR3/Nur77 regulates ER stress and induces apoptosis via interaction with TRAP gamma

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	The orphan nuclear receptor TR3/Nur77 regulates ER stress and induces apoptosis via interaction with TRAP gamma
	Chen, Hang-zi ; Wen, Quan ; Wang, Wei-jia ; He, Jian-ping ; Wu, Qiao ; Chen HZ(陈航姿) ; Wu Q(吴乔)
刊名	http://dx.doi.org/10.1016/j.biocel.2013.04.026
	2013
关键词	ENDOPLASMIC-RETICULUM STRESS UNFOLDED-PROTEIN RESPONSE CELL-DEATH TR3 TRANSLOCATION MEMBRANE NUR77 MITOCHONDRIA COMPLEX TRANSACTIVATION
英文摘要	Fundamental Research Funds for the Central Universities, China [2010121096]; Fujian Provincial Department of Science and Technology [2012J01148]; National Natural Science Foundation of China [31000620]; Ministry of Education, China [B06016]; The orphan nuclear receptor TR3 (also known as Nur77) belongs to the steroid/thyroid/retinoid nuclear receptor superfamily and plays important roles in regulating cell proliferation, differentiation and apoptosis. No physiological ligand for TR3 has been found thus far; the determination of its binding partners is therefore important to clarify the biological functions of TR3. Here, we identified translocon-associated protein subunit gamma (TRAP gamma) as a novel TR3 binding partner using a tandem affinity purification method. This interaction between TR3 and TRAP gamma was further confirmed, and the interacting regions were mapped. The ligand-binding domain of TR3 was required for TRAP gamma binding, and the C terminus of TRAP gamma was responsible for its interaction with TR3. When stimulated with 12-O-tetradecanoylphorbol 13-acetate (TPA) or CD437, this TR3-TRAP gamma interaction not only induced Ca2+ depletion in the endoplasmic reticulum (ER) but also promoted the expression of the proapoptotic transcriptional regulator CHOP. Notably, both TR3 and TRAP gamma were required for ER stress-induced apoptosis in HepG2 cells. Overall, this study demonstrated a novel, TR3-initiated signaling pathway in which TR3 regulates ER stress and induces apoptosis of hepatoma cells through its interaction with TRAP gamma. (c) 2013 Elsevier Ltd. All rights reserved.
语种	英语
出版者	PERGAMON-ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90700]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Chen, Hang-zi,Wen, Quan,Wang, Wei-jia,et al. The orphan nuclear receptor TR3/Nur77 regulates ER stress and induces apoptosis via interaction with TRAP gamma[J]. http://dx.doi.org/10.1016/j.biocel.2013.04.026,2013.
APA	Chen, Hang-zi.,Wen, Quan.,Wang, Wei-jia.,He, Jian-ping.,Wu, Qiao.,...&吴乔.(2013).The orphan nuclear receptor TR3/Nur77 regulates ER stress and induces apoptosis via interaction with TRAP gamma.http://dx.doi.org/10.1016/j.biocel.2013.04.026.
MLA	Chen, Hang-zi,et al."The orphan nuclear receptor TR3/Nur77 regulates ER stress and induces apoptosis via interaction with TRAP gamma".http://dx.doi.org/10.1016/j.biocel.2013.04.026 (2013).