A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue

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	A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue
	Xia, Xiaohu ; Xu, Ye ; Ke, Rongqin ; Zhang, Heng ; Zou, Mingqiang ; Yang, Wei ; Li, Qingge ; Xu Y(许晔) ; Li QG(李庆阁)
刊名	http://dx.doi.org/10.1007/s00216-013-7210-9
	2013
关键词	LINKED-IMMUNOSORBENT-ASSAY SILICA NANOPARTICLES CHICKEN MUSCLE MILK 2002/657/EC VALIDATION TISSUES SHRIMP HONEY ELISA
英文摘要	Commonweal Scientific Foundation for Industry of Chinese Inspection and Quarantine [201010022]; Xiamen Scientific Development Program [3502Z20055008]; National Natural Science Foundation of China [30500454]; Natural Science Foundation of Fujian Province of China [2007 J0112]; A europium nanoparticle-based lateral flow immunoassay for highly sensitive detection of chloramphenicol residue was developed. The detection result could be either qualitatively resolved with naked eye or quantitatively analyzed with the assistance of a digital camera. In the qualitative mode, the limit of detection (LOD) was found to be 0.25 ng/mL. In the quantitative mode, the half-maximal inhibition concentration (IC50) was determined to be 0.45 ng/mL and the LOD can reach an ultralow level of 0.03 ng/mL, which is similar to 100 times lower than that of the conventional colloidal gold-based lateral flow immunoassay. Potential application of the established method was demonstrated by analyzing representative cow milk samples.
语种	英语
出版者	SPRINGER HEIDELBERG
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90693]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Xia, Xiaohu,Xu, Ye,Ke, Rongqin,et al. A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue[J]. http://dx.doi.org/10.1007/s00216-013-7210-9,2013.
APA	Xia, Xiaohu.,Xu, Ye.,Ke, Rongqin.,Zhang, Heng.,Zou, Mingqiang.,...&李庆阁.(2013).A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue.http://dx.doi.org/10.1007/s00216-013-7210-9.
MLA	Xia, Xiaohu,et al."A highly sensitive europium nanoparticle-based lateral flow immunoassay for detection of chloramphenicol residue".http://dx.doi.org/10.1007/s00216-013-7210-9 (2013).