Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model

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	Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model
	Xiao, Xinye ; Luo, Pingping ; Zhao, Hui ; Chen, Jingyao ; He, Hui ; Xu, Yuxue ; Lin, Zhirong ; Zhou, Yueping ; Xu, Jianjiang ; Liu, Zuguo ; Lin ZR(林志荣) ; Zhou YP(周跃平) ; Liu ZG(刘祖国)
刊名	http://dx.doi.org/10.1016/j.exer.2013.06.005
	2013
关键词	GOBLET CELL-DENSITY SQUAMOUS METAPLASIA ALKALI BURN CYCLOSPORINE PREVALENCE TEAR TRANSPLANTATION CONJUNCTIVA POPULATION SUSPENSION
英文摘要	National Basic Research Program of China (Project 973) [2011CB504606]; National Natural Science Foundation of China, Beijing, China [81270978]; Cross-strait Program of the National Foundation [U1205025]; Human amniotic membrane (AM) is avascular but contains various beneficial bioactive factors, its extract (AE) is also effective in treating many ocular surface disorders. In this study, we for the first time evaluated the therapeutic effects of AE on dry eye induced by benzalkonium chloride in a BALB/c mouse model. Topical application of AE (1.5 and 3 mu g/eye/day) resulted in significantly longer tear break-up time on Day 3 and 6, lower fluorescein staining scores on Day 3, and lower inflammatory index on Day 6. AE reduced corneal epithelial K10 expression, inflammatory infiltration, and levels of TNF-alpha, IL-1 beta and IL-6 in BAC treated mice than that in the control mice. Moreover, decreased TUNEL positive cells in cornea and increased goblet cells in conjunctiva were also observed in AE treated corneas. Finally, AE induced more Ki-67 positive cells in corneal epithelium of dry eye mouse. Taken together, our data provide further support for BAC induced dry eye model as a valuable for dry eye study and suggest a great potential for AE as a therapeutic agent in the clinical treatment of dry eye. (C) 2013 Elsevier Ltd. All rights reserved.
语种	英语
出版者	ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90677]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Xiao, Xinye,Luo, Pingping,Zhao, Hui,et al. Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model[J]. http://dx.doi.org/10.1016/j.exer.2013.06.005,2013.
APA	Xiao, Xinye.,Luo, Pingping.,Zhao, Hui.,Chen, Jingyao.,He, Hui.,...&刘祖国.(2013).Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model.http://dx.doi.org/10.1016/j.exer.2013.06.005.
MLA	Xiao, Xinye,et al."Amniotic membrane extract ameliorates benzalkonium chloride-induced dry eye in a murine model".http://dx.doi.org/10.1016/j.exer.2013.06.005 (2013).