Progesterone and heparin-binding epidermal growth factor-like growth factor regulate the expression of tight junction protein Claudin-3 during early pregnancy

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	Progesterone and heparin-binding epidermal growth factor-like growth factor regulate the expression of tight junction protein Claudin-3 during early pregnancy
	Liang, Xuan ; Zhang, Xiu-Hong ; Han, Bing-Chen ; Lei, Wei ; Qi, Qian-Rong ; Wang, Tong-Song ; Gu, Xiao-Wei ; Yang, Zeng-Ming ; Yang ZM(杨增明)
刊名	http://dx.doi.org/10.1016/j.fertnstert.2013.07.001
	2013
关键词	EPITHELIAL-MESENCHYMAL TRANSITION EMBRYO IMPLANTATION GENE-EXPRESSION UTERINE RECEPTIVITY MOUSE UTERUS BLASTOCYST DECIDUALIZATION RECEPTOR FAMILY CANCER
英文摘要	National Basic Research Program of China [2011CB944402]; National Natural Science Foundation of China [30930013, 31071276, 31272263]; Objective: To determine Claudin-3 expression and its regulatory factors during embryo implantation. Design: Experimental mouse models and cell culture. Setting: University research laboratory. Animal(s): Sexually mature female CD-1 strain mice. Intervention(s): Ovariectomy and treatments. Main Outcome Measure(s): In situ hybridization and immunohistochemistry for detecting Claudin-3 messenger RNA and protein expression in mouse uterus, respectively; Western blot for detecting protein levels; immunofluorescence for detecting Claudin-3 protein in cultured cells. Result(s): Claudin-3 is strongly expressed in the uterine luminal epithelium on days 3 and 4 of pregnancy, and diminished at day 5 implantation sites. Then it is expressed at secondary decidual zone on day 8. Pseudopregnant uteri have a similar expression pattern as pregnant uteri from days 1-5. Claudin-3 expression is down-regulated after delayed implantation is activated by estrogen (E) treatment. Meanwhile Claudin-3 expression is stimulated by artificial decidualization. In ovariectomized mice, P induces Claudin-3 expression in the luminal epithelium, which is abrogated by P receptor antagonist RU486. Heparin-binding-epidermal growth factor (HB-EGF) downregulates Claudin-3 expression, but enhances transcription factor Snail expression. In human endometrial epithelial ECC-1 cells, both E and P could stimulate Claudin-3 expression, whereas HB-EGF decreases Claudin-3 and increases Snail expression. Conclusion(s): Claudin-3 expression in uterine luminal epithelium is stimulated by P and suppressed by HB-EGF in mice and humans. (C) 2013 by American Society for Reproductive Medicine.
语种	英语
出版者	ELSEVIER SCIENCE INC
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90662]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Liang, Xuan,Zhang, Xiu-Hong,Han, Bing-Chen,et al. Progesterone and heparin-binding epidermal growth factor-like growth factor regulate the expression of tight junction protein Claudin-3 during early pregnancy[J]. http://dx.doi.org/10.1016/j.fertnstert.2013.07.001,2013.
APA	Liang, Xuan.,Zhang, Xiu-Hong.,Han, Bing-Chen.,Lei, Wei.,Qi, Qian-Rong.,...&杨增明.(2013).Progesterone and heparin-binding epidermal growth factor-like growth factor regulate the expression of tight junction protein Claudin-3 during early pregnancy.http://dx.doi.org/10.1016/j.fertnstert.2013.07.001.
MLA	Liang, Xuan,et al."Progesterone and heparin-binding epidermal growth factor-like growth factor regulate the expression of tight junction protein Claudin-3 during early pregnancy".http://dx.doi.org/10.1016/j.fertnstert.2013.07.001 (2013).