Traceless protein delivery with an efficient recyclable nanocarrier

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	Traceless protein delivery with an efficient recyclable nanocarrier
	Wu, Xuanjun ; Song, Yunlong ; Han, Jiahuai ; Yang, Liu ; Han, Shoufa ; Han JH(韩家淮) ; Yang L(杨柳) ; Han SF(韩守法)
刊名	http://dx.doi.org/10.1039/c3bm60128e
	2013
关键词	MESOPOROUS SILICA NANOPARTICLES PHOSPHATE NANOCOMPOSITE PARTICLES CELL-PENETRATING PEPTIDES DRUG-DELIVERY IN-VIVO INTRACELLULAR DELIVERY CALCIUM-PHOSPHATE CANCER-THERAPY COMPLEX MICELLES TRANSDUCTION
英文摘要	NSF China [21272196, 21072162, 31221065, 91029304, 81061160512]; Fundamental Research Funds for the Central Universities [2011121020]; 973 program [2009CB522200]; Intracellular delivery is a prerequisite for the efficacy of many pharmaceutical proteins. Herein, vitamin B-6 (pyridoxal-5'-phosphate, PLP) functionalized calcium phosphate (CP) is used as the bio-recyclable nanocarrier for delivery of proteins into cells. Proteins could be loaded on/released from PLP-CP via formation/hydrolysis of pH sensitive aldimine bridging lysine and surface-displayed PLP. The loaded proteins could be delivered into the cytosol of HeLa, HepG2 and L929 cells where the carrier could be metabolized into endogenous metabolites of Ca2+, HPO42-, and vitamin B-6. PLP-CP mediated cell transduction is 10-40 folds more efficient than TAT which is a widely used cell penetrating peptide, demonstrating the utility of PLP-CP as the traceless platform for high-efficiency delivery of proteins into mammalian cells.
语种	英语
出版者	ROYAL SOC CHEMISTRY
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90620]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Wu, Xuanjun,Song, Yunlong,Han, Jiahuai,et al. Traceless protein delivery with an efficient recyclable nanocarrier[J]. http://dx.doi.org/10.1039/c3bm60128e,2013.
APA	Wu, Xuanjun.,Song, Yunlong.,Han, Jiahuai.,Yang, Liu.,Han, Shoufa.,...&韩守法.(2013).Traceless protein delivery with an efficient recyclable nanocarrier.http://dx.doi.org/10.1039/c3bm60128e.
MLA	Wu, Xuanjun,et al."Traceless protein delivery with an efficient recyclable nanocarrier".http://dx.doi.org/10.1039/c3bm60128e (2013).