Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment | |
Chen, Zhite ; Ao, Jingqun ; Yang, Wenchuan ; Jiao, Liping ; Zheng, Tianling ; Chen, Xinhua ; Yang WC(杨文川) ; Zheng TL(郑天凌) | |
刊名 | http://dx.doi.org/10.1007/s00253-013-4800-6 |
2013 | |
关键词 | Antigen-antibody reactions Chromatographic analysis Cloning Gene encoding Mass spectrometry Proteins Purification Sodium dodecyl sulfate Stability |
英文摘要 | A fungal strain, Penicillium chrysogenum A096, was isolated from an Arctic sediment sample. Its culture supernatant inhibited mycelial growth of some plant pathogenic fungi. After saturation of P. chrysogenum A096 culture supernatant with ammonium sulfate and ion exchange chromatography, a novel antifungal protein (Pc-Arctin) was purified and identified by matrix assisted laser desorption ionization-time of flight-time of flight-mass spectrometry (MALDI-TOF-TOF-MS). The gene encoding for Pc-Arctin consisting of 195 nucleotides was cloned from P. chrysogenum A096 to confirm the mass spectrometry result. Pc-Arctin displays antifungal activity against Paecilomyces variotii, Alternaria longipes, and Trichoderma viride at minimum inhibitory concentrations (MIC) of 24, 48, and 192 ng/disc, respectively. Pc-Arctin was most sensitive to proteinase K and then to trypsin but insensitive to papain. Pc-Arctin possesses high thermostability and cannot be antagonized by common surfactants, except for sodium dodecyl sulfate (SDS). Divalent ions, such as Mn2+, Mg2+, and Zn2+, inhibited the antifungal activity of Pc-Arctin. Hemagglutination assays showed that Pc-Arctin had no hemagglutinating or hemolytic activity against red blood cells (RBC) from rabbits, rats, and guinea pigs. Therefore, Pc-Arctin from Arctic P. chrysogenum may represent a novel antifungal protein with potential for application in controlling plant pathogenic fungal infection. ? Springer-Verlag Berlin Heidelberg 2013. |
语种 | 英语 |
出版者 | Springer Verlag |
内容类型 | 期刊论文 |
源URL | [http://dspace.xmu.edu.cn/handle/2288/90604] |
专题 | 生命科学-已发表论文 |
推荐引用方式 GB/T 7714 | Chen, Zhite,Ao, Jingqun,Yang, Wenchuan,et al. Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment[J]. http://dx.doi.org/10.1007/s00253-013-4800-6,2013. |
APA | Chen, Zhite.,Ao, Jingqun.,Yang, Wenchuan.,Jiao, Liping.,Zheng, Tianling.,...&郑天凌.(2013).Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment.http://dx.doi.org/10.1007/s00253-013-4800-6. |
MLA | Chen, Zhite,et al."Purification and characterization of a novel antifungal protein secreted by Penicillium chrysogenum from an Arctic sediment".http://dx.doi.org/10.1007/s00253-013-4800-6 (2013). |
个性服务 |
查看访问统计 |
相关权益政策 |
暂无数据 |
收藏/分享 |
除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。
修改评论