Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance

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	Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance
	Peng, XX ; Xu, CX ; Ren, HX ; Lin, XM ; Wu, L ; Wang, SY ; Wang SY(王三英)
刊名	http://dx.doi.org/10.1021/pr050159g
	2005
关键词	GRAM-NEGATIVE BACTERIA MULTIDRUG EFFLUX PUMPS MOLECULAR EPIDEMIOLOGY PERMEABILITY BARRIERS ACTIVE EFFLUX PROTEIN OPRH POLYMYXIN-B PHOP-PHOQ EXPRESSION GENE
英文摘要	Nosocomial wound infections by antibiotic-resistant Pseudomonas aeruginosa strains have increasing importance in hospitals. Outer membrane proteins of the bacterium have strong influence on its resistance to antibiotics. In the current study, a parallel proteomic approach was applied to analysis of sarcosine-insoluble outer membrane fraction of P. aeruginosa responding to ampicilin, kanamycin and tetracycline resistances. Eleven differential proteins with 15 spots were determined and then identified by MALDI-TOF/MS, in which four with increased OprF, MexA, OmpH, and decreased hypothetical protein (NCBI No. 15599856), six with increased OprF, OmpH, hypothetical protein (NCBI No. 15599183) and decreased OprG, MexA, conserved hypothetical protein (NCBI No. 15600371), and eight with increased OprF, MexA, OprL, probable Omp (NCBI No. 15599856), probable secretion protein (NCBI No. 15600167), OprD and decreased OprG, conserved hypothetical protein (NCBI No. 15600371) responded to ampicilin, kanamycin, and tetracycline resistances, respectively. With the exception of OprF, the other differential proteins did not show the same behaviors against the three antibiotic resistances. Compared with our previous report on E. coli Omps responding to ampicilin and tetracycline resistances, which was only a protein difference in quality between the two antibiotics, P. aeruginosa showed significant diversity against the three antibiotics. Our findings might provide valuable data for an understanding of antibiotic-resistant difference between different species of bacteria. Meanwhile, these proteins shared by different bacteria or a bacterium against different antibiotics may provide universal targets for the development of new drugs that control antibiotic-resistant bacteria.
语种	英语
出版者	J PROTEOME RES
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/90553]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Peng, XX,Xu, CX,Ren, HX,et al. Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance[J]. http://dx.doi.org/10.1021/pr050159g,2005.
APA	Peng, XX.,Xu, CX.,Ren, HX.,Lin, XM.,Wu, L.,...&王三英.(2005).Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance.http://dx.doi.org/10.1021/pr050159g.
MLA	Peng, XX,et al."Proteomic analysis of the sarcosine-insoluble outer membrane fraction of Pseudomonas aeruginosa responding to ampicilin, kanamycin, and tetracycline resistance".http://dx.doi.org/10.1021/pr050159g (2005).