Morphology, phylogeny and ITS-2 secondary structure of Pseudo-nitzschia brasiliana (Bacillariophyceae), including Chinese strains

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	Morphology, phylogeny and ITS-2 secondary structure of Pseudo-nitzschia brasiliana (Bacillariophyceae), including Chinese strains
	Wang, Peng ; Liang, Junrong ; Lin, Xin ; Chen, Changping ; Huang, Yingyan ; Gao, Yu ; Gao, Yahui ; Gao YH(高亚辉)
刊名	http://dx.doi.org/10.2216/09-108.1
	2012-01
关键词	SP-NOV BACILLARIOPHYCEAE RIBOSOMAL-RNA COMPLEX BACILLARIOPHYCEAE DOMOIC ACID DIATOM SEQUENCE RDNA PSEUDODELICATISSIMA HYBRIDIZATION NAPLES
英文摘要	National Natural Science Foundation of China [40831160519, 41076079, 40676080, 40876079]; national 973 project [2010CB428704]; Natural Science Foundation of Fujian Province of China [2008J0139]; The diatom Pseudo-nitzschia brasiliana Lundholm, Haste & G.A. Fryxell is documented from China for the first time. Strains isolated from southeast coastal waters were characterized using light and. electron microscopic observation. All strains were tested for domoic acid, but no toxin was detected. Genetic diversity and phylogenetic relationships of global strains of P. brasiliana were studied to assess intraspecific variation and geographic distribution patterns. Phylogenetic analyses based on the large subunit rDNA gene together with morphological observations show that P. brasiliana from China and other locations worldwide form one well-supported monophyletic clade, with P. americana as its sister. Phylogenetic analyses of internal transcribed spacer (ITS) rDNA sequences show a clear separation between five Asian strains and three European strains. In addition, ITS-2 rDNA secondary structure analyses reveal genetic variation between P. brasiliana strains from Europe and Southeast Asia, namely, a transfer of a base-pair set from a loop in the Asian group to the basis of helix II in the European group and two hemi-compensatory base changes (hemi-CBCs) in helix III between one European strain (ICMB-176) and the other strains. The occurrence of P. brasiliana in China's coastal waters is discussed.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65913]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Wang, Peng,Liang, Junrong,Lin, Xin,et al. Morphology, phylogeny and ITS-2 secondary structure of Pseudo-nitzschia brasiliana (Bacillariophyceae), including Chinese strains[J]. http://dx.doi.org/10.2216/09-108.1,2012.
APA	Wang, Peng.,Liang, Junrong.,Lin, Xin.,Chen, Changping.,Huang, Yingyan.,...&高亚辉.(2012).Morphology, phylogeny and ITS-2 secondary structure of Pseudo-nitzschia brasiliana (Bacillariophyceae), including Chinese strains.http://dx.doi.org/10.2216/09-108.1.
MLA	Wang, Peng,et al."Morphology, phylogeny and ITS-2 secondary structure of Pseudo-nitzschia brasiliana (Bacillariophyceae), including Chinese strains".http://dx.doi.org/10.2216/09-108.1 (2012).