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	Cloning and characterization of RLPK, a novel RSK-related protein kinase
	New, L. ; Zhao, M. ; Li, Y. Q. ; Bassett, W. W. ; Feng, Y. ; Ludwig, S. ; Padova, F. D. ; Gram, H. ; Han, J. H. ; Han JH(韩家淮)
	1999
关键词	P70 S6 KINASE PHORBOL ESTER 2 ISOFORMS RAT-LIVER ACTIVATION PHOSPHORYLATION PP90(RSK) RAPAMYCIN PP90RSK DOMAINS
英文摘要	A novel protein kinase whose activity can be stimulated by mitogen in vivo was cloned and characterized. The cDNA of this gene encodes an 802-amino acid protein (termed RLPK) with the highest homology (37% identity) to the two protein kinase families, p90(RSK) and p70(RSK). Like p90(RSR), but not p70(RSK), RLPK also contains two complete nonidentical protein kinase domains. RLPK mRNA is widely expressed in all human tissues examined and is enriched in the brain, heart, and placenta. In HeLa cells, transiently expressed epitope-tagged RLPK can be strongly induced by epidermal growth factor, serum, and phorbol 12-myristate 13-acetate, but only moderately up-regulated by tumor necrosis factor-ct and other stress-related stimuli. The activity of RLPK stimulated by epidermal growth factor was not inhibited by several known protein kinase C inhibitors nor by rapamycin, a known specific inhibitor for p70(RSK), but could be inhibited by herbimycin A, a tyrosine kinase inhibitor, and partially inhibited by PD98059 or SB203580, inhibitors for the mitogen-activated protein kinase pathways. Recombinant RLPK possesses high phosphorylation activity toward histone 2B and the S6 peptide, RRRLSSLRA. Although purified recombinant RLPK can be phosphorylated by ERK2 and p38 alpha in vitro, its activity is not affected by this phosphorylation. Moreover, the treatment of RLPK with acid phosphatase did not reduce its in vitro kinase activity. These data suggest that RLPK is structurally similar to previously isolated RSKs, but its regulatory mechanism may be distinct from either p70(RSK) or p90(RSK)S.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65820]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	New, L.,Zhao, M.,Li, Y. Q.,et al. Cloning and characterization of RLPK, a novel RSK-related protein kinase[J],1999.
APA	New, L..,Zhao, M..,Li, Y. Q..,Bassett, W. W..,Feng, Y..,...&韩家淮.(1999).Cloning and characterization of RLPK, a novel RSK-related protein kinase..
MLA	New, L.,et al."Cloning and characterization of RLPK, a novel RSK-related protein kinase".(1999).

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