The effect of various concentrations of papain on the properties and hydrolytic rates of beta-casein layers

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	The effect of various concentrations of papain on the properties and hydrolytic rates of beta-casein layers
	Yao, Jiangwu ; Lin, Changjian ; Tao, Tao ; Lin, Feng ; Tao T(陶涛)
刊名	http://dx.doi.org/10.1016/j.colsurfb.2012.06.030
	2013
关键词	Papain Hydrolysis beta-Casein Layer
英文摘要	Recently, much attention has been focused on the investigation of the surface biocatalysis of proteases. In this study, papain, a proteolytic enzyme was used to hydrolyze a bovine beta-casein (beta-CN) layer, which was monitored by a quartz crystal microbalance with dissipation (QCM-D). The changes of the beta-CN layers before and after hydrolysis were characterized by atomic force microscopy (AFM) imaging, grazing angle infrared spectroscopy (GA-FTIR) spectra, and contact angle measurement. Our results demonstrated that the proteolytic reaction was enzyme concentration-dependent and started with the hydrophobic C-terminal sequence of the beta-CN. The remaining beta-CN layer became thinner, smoother, stiffer, and hydrophilic after hydrolysis. These results are conducive to the further understanding of the catalysis of papain on beta-CN layers in liquid solid interfaces and the promotion of biocatalytic applications. (C) 2012 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65711]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Yao, Jiangwu,Lin, Changjian,Tao, Tao,et al. The effect of various concentrations of papain on the properties and hydrolytic rates of beta-casein layers[J]. http://dx.doi.org/10.1016/j.colsurfb.2012.06.030,2013.
APA	Yao, Jiangwu,Lin, Changjian,Tao, Tao,Lin, Feng,&陶涛.(2013).The effect of various concentrations of papain on the properties and hydrolytic rates of beta-casein layers.http://dx.doi.org/10.1016/j.colsurfb.2012.06.030.
MLA	Yao, Jiangwu,et al."The effect of various concentrations of papain on the properties and hydrolytic rates of beta-casein layers".http://dx.doi.org/10.1016/j.colsurfb.2012.06.030 (2013).