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	MEMBRANE TOPOLOGY OF A P-TYPE ATPASE - THE MGTB MAGNESIUM TRANSPORT PROTEIN OF SALMONELLA-TYPHIMURIUM
	Tao T(陶涛)
	1993
关键词	AMINO-ACID-SEQUENCE SARCOPLASMIC-RETICULUM MEMBRANE POSITIVELY CHARGED RESIDUES X-RAY-DIFFRACTION ESCHERICHIA-COLI H+-ATPASE NEUROSPORA-CRASSA TRANSMEMBRANE SEGMENTS CYTOPLASMIC LOCATION COMPLEMENTARY-DNA
英文摘要	P-type ATPases are a family of cation transport enzymes present in all species from bacteria to mammals whose members mediate membrane flux of all common biologically relevant cations. More than 50 members of this family of transporters have been sequenced; extensive structural data are available, and several members have been analyzed by site-directed mutagenesis. Nonetheless, there is no current consensus regarding their membrane topology. In this work, the Salmonella typhimurium Mg2+ transporting P-type ATPase encoded by the MgtB locus has been used as a model for P-type ATPases. Unlike other prokaryotic P-type ATPases, the MgtB protein is similar in length, amino acid sequence, and hydropathy profile to known eukaryotic P-type ATPases. The membrane topology of MgtB was analyzed by several epitope insertions in MgtB and from the activity of 35 protein fusions between MgtB and the reporter enzymes BlaM (beta-lactamase) and LacZ (beta-galactosidase). The epitope insertions within MgtB all retained function as assessed by cation uptake assays and were regulated normally by the level of Mg2+ within the growth medium. The epitope insertion and fusion protein data are completely incompatible with the numerous previously proposed models for P-type ATPases predicting 7, 8, 9, or 12 transmembrane segments. Rather, they indicate that MgtB contains 10 transmembrane segments with both amino and carboxyl termini residing within the cytosol. By extension, we suggest that all eukaryotic P-type ATPases contain 10 transmembrane segments with both termini within the cytosol.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65696]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Tao T. MEMBRANE TOPOLOGY OF A P-TYPE ATPASE - THE MGTB MAGNESIUM TRANSPORT PROTEIN OF SALMONELLA-TYPHIMURIUM[J],1993.
APA	陶涛.(1993).MEMBRANE TOPOLOGY OF A P-TYPE ATPASE - THE MGTB MAGNESIUM TRANSPORT PROTEIN OF SALMONELLA-TYPHIMURIUM..
MLA	陶涛."MEMBRANE TOPOLOGY OF A P-TYPE ATPASE - THE MGTB MAGNESIUM TRANSPORT PROTEIN OF SALMONELLA-TYPHIMURIUM".(1993).

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