Kinetics of complexing activation by the magnesium ion on green crab (Scylla serrata) alkaline phosphatase

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	Kinetics of complexing activation by the magnesium ion on green crab (Scylla serrata) alkaline phosphatase
	Park, Y. D. ; Yang, Y. ; Chen, Q. X. ; Lin, H. N. ; Liu, Q. ; Zhou, H. M. ; Chen QX(陈清西)
刊名	http://dx.doi.org/10.1139/bcb-79-6-765
	2001-12
关键词	SITE-DIRECTED MUTAGENESIS ACTIVE-SITE ZINC-BINDING METAL-IONS SEVERE HYPOPHOSPHATASIA CATALYTIC MECHANISM MUTANT ENZYME INHIBITION ARGININE-166
英文摘要	As with mammalian enzymes, green crab (Scylla serrata) alkaline phosphatase can be activated by Mg2+ through a time-dependent course. The activation is mainly a V-max effect. Tsou's method was used to study the kinetic course of activation. The results show that the enzyme was activated by a complexing scheme that had not been previously identified: the enzyme first reversibly and quickly binds Mg2+ and then undergoes a slow reversible course to activation, with a relatively high activation energy (78 +/- 4 kJ/mol) and a slow conformational change. The activation reaction is a single molecule reaction, and the apparent activation rate constant is independent of Mg2+ concentration if the concentration is sufficiently high. The microscopic rate constants of activation and the association constant were determined from the measurements. The proposed scheme may also be applied to the Mg2+ activation mechanism for mammalian enzyme, to explain why the activation rate is time-dependent and not diffusion controlled. Substrate binding was also shown to affect the activation rate constant.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65640]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Park, Y. D.,Yang, Y.,Chen, Q. X.,et al. Kinetics of complexing activation by the magnesium ion on green crab (Scylla serrata) alkaline phosphatase[J]. http://dx.doi.org/10.1139/bcb-79-6-765,2001.
APA	Park, Y. D..,Yang, Y..,Chen, Q. X..,Lin, H. N..,Liu, Q..,...&陈清西.(2001).Kinetics of complexing activation by the magnesium ion on green crab (Scylla serrata) alkaline phosphatase.http://dx.doi.org/10.1139/bcb-79-6-765.
MLA	Park, Y. D.,et al."Kinetics of complexing activation by the magnesium ion on green crab (Scylla serrata) alkaline phosphatase".http://dx.doi.org/10.1139/bcb-79-6-765 (2001).