Development of an IgM-capture ELISA for Coxsackievirus A16 infection

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	Development of an IgM-capture ELISA for Coxsackievirus A16 infection
	Xu, F. H. ; He, D. L. ; He, S. Z. ; Wu, B. ; Guan, L. ; Niu, J. J. ; Li, L. A. ; Li, C. A. ; Weng, Z. X. ; Yan, Q. A. ; Yang, L. S. ; Ge, S. X. ; Cheng, T. ; Chen, Y. X. ; Zhang, J. ; Xia, N. S. ; Chen YX(陈奕欣)
刊名	http://dx.doi.org/10.1016/j.jviromet.2010.10.009
	2011-01
关键词	ENTEROVIRUS-71 INFECTION RESPONSES
英文摘要	National Key Technology Research and Development program of China [2008BAI70B00]; Science and Technology Plan Program of Xiamen City [3502Z20093010]; National Natural Science Foundation of China [30972826]; Diagnosis of Coxsackievirus A16 (CA16) infection in China relies mainly on reverse transcription-polymerase chain reaction (RT-PCR) that require expensive equipment and special trained personnel, thus making its wide application in health care settings unlikely. In this study, a novel IgM anti-CA16 assay was developed for the detection of IgM antibodies to CA16 in serum. The responses and diagnostic value of IgM for the CA16 infection were:assessed by testing 1970 serum samples. The results showed that sensitivity of IgM test was 84.6% (259/306, 95% CI: 80.1-88.5), and specificity in control subjects and patients with CA16 HFMD was 99.2% (1508/1520, 95% CI: 98.6-99.6) and 90.3% (14/144, 95% CI: 84.2-94.6), respectively. The IgM positive rate reached 56.3% in the sera collected within the first day after onset, increased continuously to 95.3% at day 5 to day 7 after onset, and then reached 100% after more than 8 days. The cross-reaction rate in patients infected with other non-CA16 enteroviruses was 9.7% (14/144). These results suggest that the IgM anti-CA16 assay offers a rapid, convenient, and reliable method to detect acute CA16 infections. (C) 2010 Elsevier B.V. All rights reserved.
语种	英语
内容类型	期刊论文
源URL	[http://dspace.xmu.edu.cn/handle/2288/65609]
专题	生命科学－已发表论文
推荐引用方式 GB/T 7714	Xu, F. H.,He, D. L.,He, S. Z.,et al. Development of an IgM-capture ELISA for Coxsackievirus A16 infection[J]. http://dx.doi.org/10.1016/j.jviromet.2010.10.009,2011.
APA	Xu, F. H..,He, D. L..,He, S. Z..,Wu, B..,Guan, L..,...&陈奕欣.(2011).Development of an IgM-capture ELISA for Coxsackievirus A16 infection.http://dx.doi.org/10.1016/j.jviromet.2010.10.009.
MLA	Xu, F. H.,et al."Development of an IgM-capture ELISA for Coxsackievirus A16 infection".http://dx.doi.org/10.1016/j.jviromet.2010.10.009 (2011).